Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3433 and Shew_3434 are separated by 62 nucleotides Shew_3434 and Shew_3435 are separated by 276 nucleotides
Shew_3433: Shew_3433 - regulatory protein AmpE (RefSeq), at 4,099,608 to 4,100,459
_3433
Shew_3434: Shew_3434 - N-acetyl-anhydromuranmyl-L-alanine amidase (RefSeq), at 4,100,522 to 4,101,076
_3434
Shew_3435: Shew_3435 - nicotinate-nucleotide pyrophosphorylase (RefSeq), at 4,101,353 to 4,102,234
_3435
Position (kb)
4100
4101
4102 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4099.783 kb on - strand, within Shew_3433 at 4099.992 kb on + strand, within Shew_3433 at 4100.001 kb on + strand, within Shew_3433 at 4100.007 kb on + strand, within Shew_3433 at 4100.156 kb on - strand, within Shew_3433 at 4100.162 kb on + strand, within Shew_3433 at 4100.231 kb on + strand, within Shew_3433 at 4100.277 kb on - strand, within Shew_3433 at 4100.510 kb on + strand at 4100.548 kb on + strand at 4100.580 kb on + strand, within Shew_3434 at 4100.598 kb on + strand, within Shew_3434 at 4100.857 kb on + strand, within Shew_3434 at 4100.864 kb on + strand, within Shew_3434 at 4100.864 kb on + strand, within Shew_3434 at 4100.865 kb on - strand, within Shew_3434 at 4100.865 kb on - strand, within Shew_3434 at 4100.865 kb on - strand, within Shew_3434 at 4100.872 kb on - strand, within Shew_3434 at 4100.899 kb on + strand, within Shew_3434 at 4100.928 kb on + strand, within Shew_3434 at 4100.945 kb on - strand, within Shew_3434 at 4100.967 kb on - strand, within Shew_3434 at 4101.081 kb on - strand at 4101.123 kb on - strand at 4101.156 kb on - strand at 4101.209 kb on - strand at 4101.314 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 4,099,783 - Shew_3433 0.21 +0.6 4,099,992 + Shew_3433 0.45 +0.5 4,100,001 + Shew_3433 0.46 +2.4 4,100,007 + Shew_3433 0.47 +0.3 4,100,156 - Shew_3433 0.64 +0.6 4,100,162 + Shew_3433 0.65 +0.6 4,100,231 + Shew_3433 0.73 +0.2 4,100,277 - Shew_3433 0.79 +0.4 4,100,510 + -0.0 4,100,548 + +0.2 4,100,580 + Shew_3434 0.10 -1.0 4,100,598 + Shew_3434 0.14 -0.6 4,100,857 + Shew_3434 0.60 -1.0 4,100,864 + Shew_3434 0.62 -1.2 4,100,864 + Shew_3434 0.62 -0.5 4,100,865 - Shew_3434 0.62 -1.1 4,100,865 - Shew_3434 0.62 -0.8 4,100,865 - Shew_3434 0.62 -2.6 4,100,872 - Shew_3434 0.63 -1.2 4,100,899 + Shew_3434 0.68 -0.8 4,100,928 + Shew_3434 0.73 -1.5 4,100,945 - Shew_3434 0.76 -1.4 4,100,967 - Shew_3434 0.80 -1.3 4,101,081 - +1.3 4,101,123 - -0.1 4,101,156 - -0.3 4,101,209 - -0.6 4,101,314 + +1.8
Or see this region's nucleotide sequence