Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3069 and Shew_3070 are separated by 19 nucleotides Shew_3070 and Shew_3071 are separated by 357 nucleotides Shew_3071 and Shew_3072 are separated by 15 nucleotides
Shew_3069: Shew_3069 - 5-formyltetrahydrofolate cyclo-ligase (RefSeq), at 3,644,976 to 3,645,587
_3069
Shew_3070: Shew_3070 - extracellular solute-binding protein (RefSeq), at 3,645,607 to 3,646,467
_3070
Shew_3071: Shew_3071 - lipoprotein (RefSeq), at 3,646,825 to 3,647,334
_3071
Shew_3072: Shew_3072 - cold-shock protein, DNA-binding (RefSeq), at 3,647,350 to 3,647,943
_3072
Position (kb)
3645
3646
3647 Strain fitness (log2 ratio)
-2
-1
0
1 at 3645.391 kb on - strand, within Shew_3069 at 3645.689 kb on - strand at 3645.848 kb on - strand, within Shew_3070 at 3645.977 kb on - strand, within Shew_3070 at 3646.067 kb on - strand, within Shew_3070 at 3646.461 kb on - strand at 3646.531 kb on + strand at 3646.531 kb on + strand at 3646.714 kb on - strand at 3646.770 kb on + strand at 3647.017 kb on + strand, within Shew_3071 at 3647.030 kb on - strand, within Shew_3071 at 3647.214 kb on - strand, within Shew_3071 at 3647.228 kb on - strand, within Shew_3071 at 3647.286 kb on + strand at 3647.286 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 3,645,391 - Shew_3069 0.68 -0.3 3,645,689 - +0.2 3,645,848 - Shew_3070 0.28 +0.1 3,645,977 - Shew_3070 0.43 -0.9 3,646,067 - Shew_3070 0.53 -2.5 3,646,461 - -0.3 3,646,531 + +0.2 3,646,531 + -0.3 3,646,714 - +0.1 3,646,770 + +0.2 3,647,017 + Shew_3071 0.38 +0.4 3,647,030 - Shew_3071 0.40 +0.2 3,647,214 - Shew_3071 0.76 +0.4 3,647,228 - Shew_3071 0.79 +0.6 3,647,286 + -0.7 3,647,286 + +0.4
Or see this region's nucleotide sequence