Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2818 and Shew_2819 overlap by 20 nucleotides Shew_2819 and prfC are separated by 256 nucleotides prfC and Shew_2821 are separated by 195 nucleotides
Shew_2818: Shew_2818 - TatD-related deoxyribonuclease (RefSeq), at 3,339,664 to 3,340,494
_2818
Shew_2819: Shew_2819 - hypothetical protein (RefSeq), at 3,340,475 to 3,340,765
_2819
Shew_2820: prfC - peptide chain release factor 3 (RefSeq), at 3,341,022 to 3,342,602
prfC
Shew_2821: Shew_2821 - lipoprotein NlpI (RefSeq), at 3,342,798 to 3,343,688
_2821
Position (kb)
3341
3342
3343 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3340.149 kb on + strand, within Shew_2818 at 3340.308 kb on + strand, within Shew_2818 at 3340.334 kb on + strand, within Shew_2818 at 3340.334 kb on + strand, within Shew_2818 at 3340.503 kb on + strand at 3340.575 kb on - strand, within Shew_2819 at 3340.603 kb on + strand, within Shew_2819 at 3340.740 kb on - strand at 3340.778 kb on + strand at 3340.791 kb on + strand at 3341.537 kb on - strand, within prfC at 3341.712 kb on + strand, within prfC at 3342.248 kb on + strand, within prfC at 3342.796 kb on + strand at 3342.796 kb on + strand at 3343.221 kb on - strand, within Shew_2821 at 3343.300 kb on - strand, within Shew_2821 at 3343.487 kb on + strand, within Shew_2821 at 3343.487 kb on + strand, within Shew_2821 at 3343.495 kb on - strand, within Shew_2821 at 3343.573 kb on + strand, within Shew_2821
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 3,340,149 + Shew_2818 0.58 -0.1 3,340,308 + Shew_2818 0.77 +1.3 3,340,334 + Shew_2818 0.81 +2.4 3,340,334 + Shew_2818 0.81 +0.2 3,340,503 + +0.5 3,340,575 - Shew_2819 0.34 +0.4 3,340,603 + Shew_2819 0.44 +0.9 3,340,740 - +0.2 3,340,778 + +0.4 3,340,791 + -0.4 3,341,537 - prfC Shew_2820 0.33 -1.1 3,341,712 + prfC Shew_2820 0.44 -0.6 3,342,248 + prfC Shew_2820 0.78 -0.4 3,342,796 + +0.1 3,342,796 + -0.2 3,343,221 - Shew_2821 0.47 -0.9 3,343,300 - Shew_2821 0.56 +0.2 3,343,487 + Shew_2821 0.77 -0.8 3,343,487 + Shew_2821 0.77 +0.8 3,343,495 - Shew_2821 0.78 -2.6 3,343,573 + Shew_2821 0.87 -0.9
Or see this region's nucleotide sequence