Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2608 and Shew_2609 are separated by 60 nucleotides Shew_2609 and Shew_2610 overlap by 4 nucleotides Shew_2610 and Shew_2611 overlap by 1 nucleotides Shew_2611 and Shew_2612 are separated by 118 nucleotides
Shew_2608: Shew_2608 - inner-membrane translocator (RefSeq), at 3,074,392 to 3,075,465
_2608
Shew_2609: Shew_2609 - inner-membrane translocator (RefSeq), at 3,075,526 to 3,076,419
_2609
Shew_2610: Shew_2610 - AMP-dependent synthetase and ligase (RefSeq), at 3,076,416 to 3,078,383
_2610
Shew_2611: Shew_2611 - ABC transporter-related protein (RefSeq), at 3,078,383 to 3,079,180
_2611
Shew_2612: Shew_2612 - hypothetical protein (RefSeq), at 3,079,299 to 3,080,339
_2612
Position (kb)
3076
3077
3078
3079 Strain fitness (log2 ratio)
-1
0
1 at 3075.421 kb on - strand at 3075.524 kb on + strand at 3075.611 kb on + strand at 3075.763 kb on + strand, within Shew_2609 at 3076.118 kb on - strand, within Shew_2609 at 3076.343 kb on + strand at 3076.513 kb on - strand at 3077.239 kb on + strand, within Shew_2610 at 3077.384 kb on + strand, within Shew_2610 at 3077.510 kb on + strand, within Shew_2610 at 3077.542 kb on + strand, within Shew_2610 at 3077.623 kb on + strand, within Shew_2610 at 3077.631 kb on - strand, within Shew_2610 at 3077.641 kb on + strand, within Shew_2610 at 3077.715 kb on - strand, within Shew_2610 at 3078.155 kb on + strand, within Shew_2610 at 3078.423 kb on + strand at 3078.655 kb on - strand, within Shew_2611 at 3078.658 kb on + strand, within Shew_2611 at 3078.742 kb on + strand, within Shew_2611 at 3078.873 kb on + strand, within Shew_2611 at 3079.046 kb on + strand, within Shew_2611 at 3079.190 kb on + strand at 3079.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 3,075,421 - -0.9 3,075,524 + +0.4 3,075,611 + -1.1 3,075,763 + Shew_2609 0.27 -0.1 3,076,118 - Shew_2609 0.66 -0.1 3,076,343 + +1.0 3,076,513 - -0.1 3,077,239 + Shew_2610 0.42 -1.2 3,077,384 + Shew_2610 0.49 +0.1 3,077,510 + Shew_2610 0.56 -1.1 3,077,542 + Shew_2610 0.57 +0.2 3,077,623 + Shew_2610 0.61 +0.1 3,077,631 - Shew_2610 0.62 +0.2 3,077,641 + Shew_2610 0.62 +0.1 3,077,715 - Shew_2610 0.66 -0.4 3,078,155 + Shew_2610 0.88 -0.4 3,078,423 + -1.3 3,078,655 - Shew_2611 0.34 -0.4 3,078,658 + Shew_2611 0.34 +0.4 3,078,742 + Shew_2611 0.45 -1.1 3,078,873 + Shew_2611 0.61 +1.4 3,079,046 + Shew_2611 0.83 -0.9 3,079,190 + -0.4 3,079,190 - +0.0
Or see this region's nucleotide sequence