Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2592 and Shew_2593 are separated by 214 nucleotides Shew_2593 and Shew_2594 are separated by 108 nucleotides Shew_2594 and Shew_2595 are separated by 147 nucleotides
Shew_2592: Shew_2592 - peptidase M28 (RefSeq), at 3,057,319 to 3,058,905
_2592
Shew_2593: Shew_2593 - acyl-CoA synthetase (RefSeq), at 3,059,120 to 3,060,844
_2593
Shew_2594: Shew_2594 - hypothetical protein (RefSeq), at 3,060,953 to 3,061,225
_2594
Shew_2595: Shew_2595 - hypothetical protein (RefSeq), at 3,061,373 to 3,061,678
_2595
Position (kb)
3059
3060
3061 Strain fitness (log2 ratio)
-1
0
1 at 3058.692 kb on - strand, within Shew_2592 at 3058.856 kb on + strand at 3059.729 kb on - strand, within Shew_2593 at 3059.811 kb on - strand, within Shew_2593 at 3059.816 kb on + strand, within Shew_2593 at 3059.824 kb on - strand, within Shew_2593 at 3059.893 kb on + strand, within Shew_2593 at 3060.014 kb on - strand, within Shew_2593 at 3060.028 kb on + strand, within Shew_2593 at 3060.212 kb on - strand, within Shew_2593 at 3060.858 kb on + strand at 3061.016 kb on + strand, within Shew_2594 at 3061.043 kb on + strand, within Shew_2594 at 3061.195 kb on + strand, within Shew_2594 at 3061.323 kb on + strand at 3061.381 kb on + strand at 3061.497 kb on + strand, within Shew_2595 at 3061.507 kb on + strand, within Shew_2595 at 3061.642 kb on - strand, within Shew_2595 at 3061.653 kb on + strand at 3061.661 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 3,058,692 - Shew_2592 0.87 -0.2 3,058,856 + +0.1 3,059,729 - Shew_2593 0.35 -0.3 3,059,811 - Shew_2593 0.40 -0.5 3,059,816 + Shew_2593 0.40 +0.7 3,059,824 - Shew_2593 0.41 -0.2 3,059,893 + Shew_2593 0.45 +1.5 3,060,014 - Shew_2593 0.52 -1.4 3,060,028 + Shew_2593 0.53 -0.9 3,060,212 - Shew_2593 0.63 +0.1 3,060,858 + +0.9 3,061,016 + Shew_2594 0.23 -0.1 3,061,043 + Shew_2594 0.33 -0.3 3,061,195 + Shew_2594 0.89 +0.0 3,061,323 + +0.1 3,061,381 + +0.1 3,061,497 + Shew_2595 0.41 +0.6 3,061,507 + Shew_2595 0.44 +1.1 3,061,642 - Shew_2595 0.88 +0.5 3,061,653 + -0.1 3,061,661 - +0.1
Or see this region's nucleotide sequence