Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2493 and Shew_2494 are separated by 89 nucleotides Shew_2494 and pspB overlap by 14 nucleotides pspB and Shew_2496 are separated by 20 nucleotides Shew_2496 and Shew_2497 are separated by 177 nucleotides
Shew_2493: Shew_2493 - protein of unknown function DUF463, YcjX family protein (RefSeq), at 2,933,058 to 2,934,503
_2493
Shew_2494: Shew_2494 - phage shock protein C, PspC (RefSeq), at 2,934,593 to 2,934,988
_2494
Shew_2495: pspB - phage shock protein B (RefSeq), at 2,934,975 to 2,935,214
pspB
Shew_2496: Shew_2496 - phage shock protein A, PspA (RefSeq), at 2,935,235 to 2,935,918
_2496
Shew_2497: Shew_2497 - sigma-54 dependent trancsriptional regulator (RefSeq), at 2,936,096 to 2,937,166
_2497
Position (kb)
2935
2936 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2934.524 kb on + strand at 2934.613 kb on + strand at 2934.636 kb on + strand, within Shew_2494 at 2934.926 kb on + strand, within Shew_2494 at 2935.299 kb on - strand at 2935.360 kb on + strand, within Shew_2496 at 2935.611 kb on - strand, within Shew_2496 at 2935.611 kb on - strand, within Shew_2496 at 2935.682 kb on - strand, within Shew_2496 at 2935.953 kb on + strand at 2936.122 kb on + strand at 2936.130 kb on - strand at 2936.130 kb on - strand at 2936.142 kb on - strand at 2936.142 kb on - strand at 2936.249 kb on + strand, within Shew_2497 at 2936.436 kb on + strand, within Shew_2497 at 2936.436 kb on + strand, within Shew_2497 at 2936.444 kb on - strand, within Shew_2497 at 2936.517 kb on + strand, within Shew_2497 at 2936.810 kb on + strand, within Shew_2497
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 2,934,524 + -0.2 2,934,613 + -0.1 2,934,636 + Shew_2494 0.11 -0.3 2,934,926 + Shew_2494 0.84 +0.7 2,935,299 - -0.4 2,935,360 + Shew_2496 0.18 -1.2 2,935,611 - Shew_2496 0.55 -2.0 2,935,611 - Shew_2496 0.55 +2.6 2,935,682 - Shew_2496 0.65 -2.0 2,935,953 + -0.8 2,936,122 + -0.4 2,936,130 - +0.3 2,936,130 - -0.1 2,936,142 - +0.1 2,936,142 - -0.5 2,936,249 + Shew_2497 0.14 +0.8 2,936,436 + Shew_2497 0.32 -0.5 2,936,436 + Shew_2497 0.32 +0.5 2,936,444 - Shew_2497 0.32 -0.4 2,936,517 + Shew_2497 0.39 -1.1 2,936,810 + Shew_2497 0.67 +0.3
Or see this region's nucleotide sequence