Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1924 and Shew_1925 are separated by 183 nucleotides Shew_1925 and Shew_1926 are separated by 3 nucleotides Shew_1926 and Shew_1927 are separated by 11 nucleotides
Shew_1924: Shew_1924 - succinylglutamate desuccinylase (RefSeq), at 2,221,280 to 2,222,347
_1924
Shew_1925: Shew_1925 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (RefSeq), at 2,222,531 to 2,223,709
_1925
Shew_1926: Shew_1926 - transketolase, central region (RefSeq), at 2,223,713 to 2,224,690
_1926
Shew_1927: Shew_1927 - dihydrolipoamide acetyltransferase (RefSeq), at 2,224,702 to 2,226,264
_1927
Position (kb)
2222
2223
2224 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2222.301 kb on + strand at 2222.404 kb on + strand at 2222.869 kb on - strand, within Shew_1925 at 2222.883 kb on + strand, within Shew_1925 at 2223.060 kb on - strand, within Shew_1925 at 2223.096 kb on + strand, within Shew_1925 at 2223.119 kb on + strand, within Shew_1925 at 2223.127 kb on - strand, within Shew_1925 at 2223.127 kb on - strand, within Shew_1925 at 2223.334 kb on + strand, within Shew_1925 at 2223.363 kb on + strand, within Shew_1925 at 2223.831 kb on - strand, within Shew_1926 at 2223.842 kb on - strand, within Shew_1926 at 2224.075 kb on - strand, within Shew_1926
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 2,222,301 + +1.2 2,222,404 + -0.6 2,222,869 - Shew_1925 0.29 -0.9 2,222,883 + Shew_1925 0.30 -1.3 2,223,060 - Shew_1925 0.45 -3.2 2,223,096 + Shew_1925 0.48 +2.2 2,223,119 + Shew_1925 0.50 -0.7 2,223,127 - Shew_1925 0.51 -0.8 2,223,127 - Shew_1925 0.51 -2.3 2,223,334 + Shew_1925 0.68 +1.6 2,223,363 + Shew_1925 0.71 -0.9 2,223,831 - Shew_1926 0.12 -2.2 2,223,842 - Shew_1926 0.13 +0.6 2,224,075 - Shew_1926 0.37 -0.7
Or see this region's nucleotide sequence