Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1539 and Shew_1540 are separated by 200 nucleotides Shew_1540 and Shew_1541 are separated by 60 nucleotides Shew_1541 and Shew_1542 are separated by 22 nucleotides
Shew_1539: Shew_1539 - LysR family transcriptional regulator (RefSeq), at 1,767,142 to 1,768,011
_1539
Shew_1540: Shew_1540 - alcohol dehydrogenase (RefSeq), at 1,768,212 to 1,769,342
_1540
Shew_1541: Shew_1541 - carboxylesterase (RefSeq), at 1,769,403 to 1,770,239
_1541
Shew_1542: Shew_1542 - major facilitator transporter (RefSeq), at 1,770,262 to 1,771,800
_1542
Position (kb)
1768
1769
1770 Strain fitness (log2 ratio)
-1
0
1 at 1767.470 kb on - strand, within Shew_1539 at 1767.480 kb on - strand, within Shew_1539 at 1768.006 kb on - strand at 1768.214 kb on - strand at 1768.653 kb on + strand, within Shew_1540 at 1768.682 kb on + strand, within Shew_1540 at 1768.688 kb on + strand, within Shew_1540 at 1769.111 kb on - strand, within Shew_1540 at 1769.185 kb on + strand, within Shew_1540 at 1769.195 kb on + strand, within Shew_1540 at 1769.859 kb on + strand, within Shew_1541 at 1770.059 kb on + strand, within Shew_1541 at 1770.167 kb on - strand at 1770.183 kb on + strand at 1770.313 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 1,767,470 - Shew_1539 0.38 +0.5 1,767,480 - Shew_1539 0.39 +0.4 1,768,006 - +0.1 1,768,214 - -0.0 1,768,653 + Shew_1540 0.39 +1.1 1,768,682 + Shew_1540 0.42 -0.1 1,768,688 + Shew_1540 0.42 +1.6 1,769,111 - Shew_1540 0.79 -0.1 1,769,185 + Shew_1540 0.86 -0.2 1,769,195 + Shew_1540 0.87 +0.2 1,769,859 + Shew_1541 0.54 -0.2 1,770,059 + Shew_1541 0.78 +0.0 1,770,167 - +0.9 1,770,183 + +0.9 1,770,313 + -0.1
Or see this region's nucleotide sequence