Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1210 and Shew_1211 overlap by 19 nucleotides Shew_1211 and Shew_1212 are separated by 49 nucleotides Shew_1212 and Shew_1213 are separated by 77 nucleotides
Shew_1210: Shew_1210 - stationary-phase survival protein SurE (RefSeq), at 1,404,368 to 1,405,117
_1210
Shew_1211: Shew_1211 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,405,099 to 1,405,749
_1211
Shew_1212: Shew_1212 - peptidase M23B (RefSeq), at 1,405,799 to 1,406,731
_1212
Shew_1213: Shew_1213 - RpoD family RNA polymerase sigma factor (RefSeq), at 1,406,809 to 1,407,783
_1213
Position (kb) 1405
1406
1407 Strain fitness (log2 ratio) -1
0
1 at 1405.267 kb on + strand, within Shew_1211 at 1405.302 kb on + strand, within Shew_1211 at 1405.310 kb on - strand, within Shew_1211 at 1405.544 kb on + strand, within Shew_1211 at 1405.995 kb on + strand, within Shew_1212 at 1406.079 kb on - strand, within Shew_1212 at 1406.562 kb on + strand, within Shew_1212 at 1406.833 kb on - strand at 1406.891 kb on + strand at 1406.915 kb on + strand, within Shew_1213 at 1406.929 kb on + strand, within Shew_1213
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 1,405,267 + Shew_1211 0.26 +0.0 1,405,302 + Shew_1211 0.31 +0.8 1,405,310 - Shew_1211 0.32 -1.1 1,405,544 + Shew_1211 0.68 -0.6 1,405,995 + Shew_1212 0.21 -0.9 1,406,079 - Shew_1212 0.30 -0.9 1,406,562 + Shew_1212 0.82 -0.5 1,406,833 - +0.4 1,406,891 + -0.3 1,406,915 + Shew_1213 0.11 +0.5 1,406,929 + Shew_1213 0.12 +1.0
Or see this region's nucleotide sequence