Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0713 and cobT are separated by 122 nucleotides cobT and Shew_0715 are separated by 41 nucleotides Shew_0715 and Shew_0716 overlap by 4 nucleotides
Shew_0713: Shew_0713 - transport system permease protein (RefSeq), at 813,329 to 814,372
_0713
Shew_0714: cobT - nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (RefSeq), at 814,495 to 815,523
cobT
Shew_0715: Shew_0715 - cobalamin 5'-phosphate synthase (RefSeq), at 815,565 to 816,332
_0715
Shew_0716: Shew_0716 - cobalbumin biosynthesis enzyme (RefSeq), at 816,329 to 816,877
_0716
Position (kb) 814
815
816 Strain fitness (log2 ratio) -2
-1
0
1 at 813.657 kb on + strand, within Shew_0713 at 813.726 kb on + strand, within Shew_0713 at 813.891 kb on - strand, within Shew_0713 at 813.909 kb on + strand, within Shew_0713 at 813.914 kb on + strand, within Shew_0713 at 813.917 kb on - strand, within Shew_0713 at 813.927 kb on - strand, within Shew_0713 at 814.056 kb on + strand, within Shew_0713 at 814.781 kb on - strand, within cobT at 814.879 kb on + strand, within cobT at 815.578 kb on + strand at 815.720 kb on + strand, within Shew_0715 at 815.728 kb on - strand, within Shew_0715 at 815.944 kb on - strand, within Shew_0715 at 816.002 kb on - strand, within Shew_0715 at 816.079 kb on - strand, within Shew_0715 at 816.304 kb on - strand at 816.411 kb on + strand, within Shew_0716 at 816.485 kb on + strand, within Shew_0716 at 816.518 kb on + strand, within Shew_0716
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 813,657 + Shew_0713 0.31 +0.3 813,726 + Shew_0713 0.38 -1.0 813,891 - Shew_0713 0.54 +0.0 813,909 + Shew_0713 0.56 +0.4 813,914 + Shew_0713 0.56 -0.2 813,917 - Shew_0713 0.56 +0.6 813,927 - Shew_0713 0.57 +0.3 814,056 + Shew_0713 0.70 +0.1 814,781 - cobT Shew_0714 0.28 +0.2 814,879 + cobT Shew_0714 0.37 +0.7 815,578 + -0.5 815,720 + Shew_0715 0.20 -0.4 815,728 - Shew_0715 0.21 -1.8 815,944 - Shew_0715 0.49 +0.2 816,002 - Shew_0715 0.57 -0.2 816,079 - Shew_0715 0.67 +0.1 816,304 - +0.7 816,411 + Shew_0716 0.15 -1.1 816,485 + Shew_0716 0.28 -1.4 816,518 + Shew_0716 0.34 +0.3
Or see this region's nucleotide sequence