Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0201 and argC are separated by 273 nucleotides argC and Shew_0203 are separated by 28 nucleotides Shew_0203 and Shew_0204 are separated by 42 nucleotides
Shew_0201: Shew_0201 - acetylornithine deacetylase (RefSeq), at 213,864 to 215,024
_0201
Shew_0202: argC - N-acetyl-gamma-glutamyl-phosphate reductase (RefSeq), at 215,298 to 216,278
argC
Shew_0203: Shew_0203 - acetylglutamate kinase (RefSeq), at 216,307 to 217,092
_0203
Shew_0204: Shew_0204 - ornithine carbamoyltransferase (RefSeq), at 217,135 to 218,040
_0204
Position (kb)
215
216
217 Strain fitness (log2 ratio)
-1
0
1 at 214.549 kb on + strand, within Shew_0201 at 214.549 kb on + strand, within Shew_0201 at 214.557 kb on - strand, within Shew_0201 at 214.557 kb on - strand, within Shew_0201 at 214.557 kb on - strand, within Shew_0201 at 214.557 kb on - strand, within Shew_0201 at 214.557 kb on - strand, within Shew_0201 at 214.557 kb on - strand, within Shew_0201 at 214.741 kb on - strand, within Shew_0201 at 215.553 kb on + strand, within argC at 215.606 kb on + strand, within argC at 215.772 kb on + strand, within argC at 216.029 kb on + strand, within argC at 216.037 kb on - strand, within argC at 216.037 kb on - strand, within argC at 216.075 kb on - strand, within argC at 216.098 kb on - strand, within argC at 216.145 kb on + strand, within argC at 216.358 kb on - strand at 216.360 kb on + strand at 216.465 kb on + strand, within Shew_0203 at 216.473 kb on - strand, within Shew_0203 at 216.495 kb on + strand, within Shew_0203 at 216.511 kb on - strand, within Shew_0203 at 216.706 kb on + strand, within Shew_0203 at 216.786 kb on + strand, within Shew_0203 at 216.865 kb on + strand, within Shew_0203 at 216.908 kb on - strand, within Shew_0203 at 216.914 kb on + strand, within Shew_0203 at 216.932 kb on - strand, within Shew_0203 at 216.957 kb on + strand, within Shew_0203 at 216.957 kb on + strand, within Shew_0203 at 216.963 kb on + strand, within Shew_0203 at 216.973 kb on + strand, within Shew_0203 at 217.077 kb on + strand at 217.263 kb on - strand, within Shew_0204
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 214,549 + Shew_0201 0.59 +0.2 214,549 + Shew_0201 0.59 -0.8 214,557 - Shew_0201 0.60 -0.9 214,557 - Shew_0201 0.60 -0.2 214,557 - Shew_0201 0.60 -0.3 214,557 - Shew_0201 0.60 +0.0 214,557 - Shew_0201 0.60 +0.4 214,557 - Shew_0201 0.60 -0.0 214,741 - Shew_0201 0.76 -0.0 215,553 + argC Shew_0202 0.26 +0.5 215,606 + argC Shew_0202 0.31 +0.5 215,772 + argC Shew_0202 0.48 +1.2 216,029 + argC Shew_0202 0.75 +0.4 216,037 - argC Shew_0202 0.75 +0.4 216,037 - argC Shew_0202 0.75 +0.4 216,075 - argC Shew_0202 0.79 +0.8 216,098 - argC Shew_0202 0.82 +0.7 216,145 + argC Shew_0202 0.86 +1.0 216,358 - +0.1 216,360 + +0.4 216,465 + Shew_0203 0.20 +0.5 216,473 - Shew_0203 0.21 +0.2 216,495 + Shew_0203 0.24 +0.6 216,511 - Shew_0203 0.26 +0.5 216,706 + Shew_0203 0.51 +0.2 216,786 + Shew_0203 0.61 +0.5 216,865 + Shew_0203 0.71 +0.3 216,908 - Shew_0203 0.76 -0.0 216,914 + Shew_0203 0.77 +0.7 216,932 - Shew_0203 0.80 -0.4 216,957 + Shew_0203 0.83 +0.8 216,957 + Shew_0203 0.83 +0.4 216,963 + Shew_0203 0.83 +0.7 216,973 + Shew_0203 0.85 +0.3 217,077 + +1.0 217,263 - Shew_0204 0.14 +0.3
Or see this region's nucleotide sequence