Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0023 and Shew_0024 are separated by 21 nucleotides Shew_0024 and Shew_0025 are separated by 272 nucleotides Shew_0025 and Shew_0026 are separated by 115 nucleotides Shew_0026 and Shew_0027 are separated by 12 nucleotides
Shew_0023: Shew_0023 - protoporphyrinogen oxidase (RefSeq), at 27,056 to 27,583
_0023
Shew_0024: Shew_0024 - ArsR family transcriptional regulator (RefSeq), at 27,605 to 27,910
_0024
Shew_0025: Shew_0025 - protoporphyrinogen oxidase (RefSeq), at 28,183 to 28,716
_0025
Shew_0026: Shew_0026 - hypothetical protein (RefSeq), at 28,832 to 29,368
_0026
Shew_0027: Shew_0027 - glutathione S-transferase domain-containing protein (RefSeq), at 29,381 to 30,028
_0027
Position (kb)
28
29 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 27.208 kb on + strand, within Shew_0023 at 27.420 kb on - strand, within Shew_0023 at 27.540 kb on + strand at 27.556 kb on - strand at 27.567 kb on + strand at 27.594 kb on - strand at 27.671 kb on + strand, within Shew_0024 at 27.851 kb on - strand, within Shew_0024 at 28.301 kb on + strand, within Shew_0025 at 28.365 kb on - strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.442 kb on + strand, within Shew_0025 at 28.700 kb on - strand at 28.750 kb on + strand at 29.027 kb on - strand, within Shew_0026 at 29.135 kb on + strand, within Shew_0026 at 29.328 kb on - strand at 29.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 27,208 + Shew_0023 0.29 +0.1 27,420 - Shew_0023 0.69 +0.8 27,540 + +0.7 27,556 - +0.6 27,567 + +0.9 27,594 - +0.0 27,671 + Shew_0024 0.22 -0.6 27,851 - Shew_0024 0.80 -2.2 28,301 + Shew_0025 0.22 -0.1 28,365 - Shew_0025 0.34 +0.3 28,401 + Shew_0025 0.41 -0.1 28,401 + Shew_0025 0.41 -0.9 28,401 + Shew_0025 0.41 +0.3 28,442 + Shew_0025 0.49 -1.4 28,700 - +0.0 28,750 + -3.6 29,027 - Shew_0026 0.36 +1.3 29,135 + Shew_0026 0.56 +0.2 29,328 - -0.6 29,371 - -0.2
Or see this region's nucleotide sequence