Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2742 and Dshi_2743 are separated by 200 nucleotides Dshi_2743 and Dshi_2744 are separated by 191 nucleotides Dshi_2744 and Dshi_2745 are separated by 60 nucleotides Dshi_2745 and Dshi_2746 are separated by 31 nucleotides
Dshi_2742: Dshi_2742 - Adenylosuccinate synthase (RefSeq), at 2,903,816 to 2,905,111
_2742
Dshi_2743: Dshi_2743 - preprotein translocase, SecG subunit (RefSeq), at 2,905,312 to 2,905,680
_2743
Dshi_2744: Dshi_2744 - CTP synthase (RefSeq), at 2,905,872 to 2,907,515
_2744
Dshi_2745: Dshi_2745 - phenazine biosynthesis protein PhzF family (RefSeq), at 2,907,576 to 2,908,436
_2745
Dshi_2746: Dshi_2746 - hypothetical protein (RefSeq), at 2,908,468 to 2,908,728
_2746
Position (kb)
2905
2906
2907
2908 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2905.235 kb on + strand at 2907.610 kb on + strand at 2907.677 kb on + strand, within Dshi_2745 at 2907.824 kb on + strand, within Dshi_2745 at 2907.921 kb on - strand, within Dshi_2745 at 2907.935 kb on - strand, within Dshi_2745 at 2907.939 kb on + strand, within Dshi_2745 at 2908.113 kb on + strand, within Dshi_2745 at 2908.397 kb on + strand at 2908.466 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 2,905,235 + +0.8 2,907,610 + -1.1 2,907,677 + Dshi_2745 0.12 -0.5 2,907,824 + Dshi_2745 0.29 +2.0 2,907,921 - Dshi_2745 0.40 +0.5 2,907,935 - Dshi_2745 0.42 -1.3 2,907,939 + Dshi_2745 0.42 -2.7 2,908,113 + Dshi_2745 0.62 -1.9 2,908,397 + -1.5 2,908,466 + -1.1
Or see this region's nucleotide sequence