Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2573 and Dshi_2574 are separated by 270 nucleotides Dshi_2574 and Dshi_2575 are separated by 88 nucleotides Dshi_2575 and Dshi_2576 overlap by 4 nucleotides Dshi_2576 and Dshi_2577 are separated by 100 nucleotides
Dshi_2573: Dshi_2573 - hypothetical protein (RefSeq), at 2,740,015 to 2,740,470
_2573
Dshi_2574: Dshi_2574 - CoA-binding domain protein (RefSeq), at 2,740,741 to 2,741,226
_2574
Dshi_2575: Dshi_2575 - phosphoribosyl-ATP pyrophosphohydrolase (RefSeq), at 2,741,315 to 2,741,623
_2575
Dshi_2576: Dshi_2576 - imidazoleglycerol phosphate synthase, cyclase subunit (RefSeq), at 2,741,620 to 2,742,381
_2576
Dshi_2577: Dshi_2577 - putative RNA methylase (RefSeq), at 2,742,482 to 2,743,606
_2577
Position (kb)
2741
2742 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2740.325 kb on - strand, within Dshi_2573 at 2740.425 kb on - strand at 2740.459 kb on + strand at 2740.459 kb on + strand at 2740.467 kb on - strand at 2740.478 kb on + strand at 2740.478 kb on + strand at 2740.486 kb on - strand at 2740.502 kb on + strand at 2740.554 kb on - strand at 2740.607 kb on + strand at 2740.683 kb on - strand at 2740.815 kb on - strand, within Dshi_2574 at 2741.201 kb on - strand at 2741.213 kb on + strand at 2742.564 kb on + strand at 2742.619 kb on - strand, within Dshi_2577
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 2,740,325 - Dshi_2573 0.68 +2.9 2,740,425 - -1.0 2,740,459 + -1.5 2,740,459 + +0.3 2,740,467 - -1.9 2,740,478 + -0.7 2,740,478 + -1.3 2,740,486 - -0.8 2,740,502 + -0.8 2,740,554 - -0.6 2,740,607 + +0.0 2,740,683 - -0.9 2,740,815 - Dshi_2574 0.15 -0.3 2,741,201 - -0.2 2,741,213 + -1.4 2,742,564 + -0.7 2,742,619 - Dshi_2577 0.12 -2.9
Or see this region's nucleotide sequence