Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2275 and Dshi_R0042 are separated by 81 nucleotides Dshi_R0042 and Dshi_2276 are separated by 300 nucleotides Dshi_2276 and Dshi_2277 overlap by 4 nucleotides Dshi_2277 and Dshi_2278 are separated by 84 nucleotides
Dshi_2275: Dshi_2275 - single-stranded-DNA-specific exonuclease RecJ (RefSeq), at 2,398,150 to 2,399,907
_2275
Dshi_R0042: Dshi_R0042 - tRNA-Glu (RefSeq), at 2,399,989 to 2,400,063
_R0042
Dshi_2276: Dshi_2276 - hypothetical protein (RefSeq), at 2,400,364 to 2,400,894
_2276
Dshi_2277: Dshi_2277 - molybdenum cofactor synthesis domain (RefSeq), at 2,400,891 to 2,402,162
_2277
Dshi_2278: Dshi_2278 - molybdopterin oxidoreductase (RefSeq), at 2,402,247 to 2,404,919
_2278
Position (kb)
2400
2401
2402
2403 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2399.893 kb on - strand at 2400.145 kb on - strand at 2400.223 kb on + strand at 2400.273 kb on - strand at 2400.335 kb on + strand at 2400.382 kb on + strand at 2400.414 kb on - strand at 2400.425 kb on - strand, within Dshi_2276 at 2400.504 kb on - strand, within Dshi_2276 at 2400.506 kb on + strand, within Dshi_2276 at 2400.509 kb on - strand, within Dshi_2276 at 2400.514 kb on - strand, within Dshi_2276 at 2400.514 kb on - strand, within Dshi_2276 at 2400.584 kb on + strand, within Dshi_2276 at 2400.592 kb on - strand, within Dshi_2276 at 2400.602 kb on - strand, within Dshi_2276 at 2400.645 kb on + strand, within Dshi_2276 at 2400.647 kb on + strand, within Dshi_2276 at 2400.647 kb on + strand, within Dshi_2276 at 2400.647 kb on + strand, within Dshi_2276 at 2400.663 kb on - strand, within Dshi_2276 at 2400.668 kb on - strand, within Dshi_2276 at 2400.669 kb on + strand, within Dshi_2276 at 2400.724 kb on - strand, within Dshi_2276 at 2400.728 kb on + strand, within Dshi_2276 at 2400.732 kb on + strand, within Dshi_2276 at 2400.732 kb on + strand, within Dshi_2276 at 2400.733 kb on + strand, within Dshi_2276 at 2400.740 kb on - strand, within Dshi_2276 at 2400.750 kb on + strand, within Dshi_2276 at 2400.778 kb on - strand, within Dshi_2276 at 2400.783 kb on - strand, within Dshi_2276 at 2400.783 kb on - strand, within Dshi_2276 at 2400.795 kb on + strand, within Dshi_2276 at 2400.810 kb on + strand, within Dshi_2276 at 2400.810 kb on + strand, within Dshi_2276 at 2400.810 kb on + strand, within Dshi_2276 at 2400.818 kb on - strand, within Dshi_2276 at 2400.920 kb on - strand at 2400.927 kb on + strand at 2400.927 kb on + strand at 2400.927 kb on + strand at 2400.950 kb on + strand at 2400.950 kb on + strand at 2400.951 kb on - strand at 2401.040 kb on + strand, within Dshi_2277 at 2401.040 kb on + strand, within Dshi_2277 at 2401.116 kb on + strand, within Dshi_2277 at 2401.139 kb on - strand, within Dshi_2277 at 2401.150 kb on - strand, within Dshi_2277 at 2401.182 kb on - strand, within Dshi_2277 at 2401.239 kb on - strand, within Dshi_2277 at 2401.304 kb on - strand, within Dshi_2277 at 2401.308 kb on + strand, within Dshi_2277 at 2401.308 kb on + strand, within Dshi_2277 at 2401.311 kb on - strand, within Dshi_2277 at 2401.316 kb on - strand, within Dshi_2277 at 2401.334 kb on + strand, within Dshi_2277 at 2401.347 kb on + strand, within Dshi_2277 at 2401.347 kb on + strand, within Dshi_2277 at 2401.406 kb on + strand, within Dshi_2277 at 2401.472 kb on + strand, within Dshi_2277 at 2401.472 kb on + strand, within Dshi_2277 at 2401.473 kb on - strand, within Dshi_2277 at 2401.497 kb on + strand, within Dshi_2277 at 2401.535 kb on - strand, within Dshi_2277 at 2401.537 kb on + strand, within Dshi_2277 at 2401.538 kb on + strand, within Dshi_2277 at 2401.603 kb on + strand, within Dshi_2277 at 2401.613 kb on + strand, within Dshi_2277 at 2401.627 kb on + strand, within Dshi_2277 at 2401.687 kb on + strand, within Dshi_2277 at 2401.688 kb on - strand, within Dshi_2277 at 2401.747 kb on + strand, within Dshi_2277 at 2401.914 kb on + strand, within Dshi_2277 at 2401.922 kb on - strand, within Dshi_2277 at 2401.929 kb on + strand, within Dshi_2277 at 2401.957 kb on - strand, within Dshi_2277 at 2401.995 kb on + strand, within Dshi_2277 at 2402.009 kb on + strand, within Dshi_2277 at 2402.029 kb on - strand, within Dshi_2277 at 2402.086 kb on + strand at 2402.092 kb on - strand at 2402.094 kb on - strand at 2402.139 kb on - strand at 2402.231 kb on + strand at 2402.243 kb on + strand at 2402.252 kb on - strand at 2402.291 kb on - strand at 2402.339 kb on + strand at 2402.352 kb on - strand at 2402.395 kb on - strand at 2402.433 kb on + strand at 2402.441 kb on - strand at 2402.464 kb on + strand at 2402.486 kb on + strand at 2402.488 kb on - strand at 2402.494 kb on - strand at 2402.550 kb on + strand, within Dshi_2278 at 2402.593 kb on + strand, within Dshi_2278 at 2402.601 kb on - strand, within Dshi_2278 at 2402.601 kb on - strand, within Dshi_2278 at 2402.624 kb on - strand, within Dshi_2278 at 2402.660 kb on + strand, within Dshi_2278 at 2402.660 kb on + strand, within Dshi_2278 at 2402.660 kb on + strand, within Dshi_2278 at 2402.665 kb on + strand, within Dshi_2278 at 2402.668 kb on - strand, within Dshi_2278 at 2402.668 kb on - strand, within Dshi_2278 at 2402.668 kb on - strand, within Dshi_2278 at 2402.678 kb on - strand, within Dshi_2278 at 2402.691 kb on - strand, within Dshi_2278 at 2402.712 kb on + strand, within Dshi_2278 at 2402.838 kb on + strand, within Dshi_2278 at 2402.857 kb on + strand, within Dshi_2278 at 2402.864 kb on - strand, within Dshi_2278 at 2402.894 kb on + strand, within Dshi_2278 at 2402.894 kb on + strand, within Dshi_2278 at 2402.924 kb on + strand, within Dshi_2278 at 2402.928 kb on + strand, within Dshi_2278 at 2402.936 kb on - strand, within Dshi_2278 at 2402.961 kb on - strand, within Dshi_2278 at 2403.082 kb on + strand, within Dshi_2278 at 2403.091 kb on - strand, within Dshi_2278 at 2403.130 kb on + strand, within Dshi_2278 at 2403.140 kb on + strand, within Dshi_2278
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 2,399,893 - -2.0 2,400,145 - -1.1 2,400,223 + +1.5 2,400,273 - +0.2 2,400,335 + -0.4 2,400,382 + -0.8 2,400,414 - -0.6 2,400,425 - Dshi_2276 0.11 -0.7 2,400,504 - Dshi_2276 0.26 -1.4 2,400,506 + Dshi_2276 0.27 -1.3 2,400,509 - Dshi_2276 0.27 +0.2 2,400,514 - Dshi_2276 0.28 -1.2 2,400,514 - Dshi_2276 0.28 -0.8 2,400,584 + Dshi_2276 0.41 -1.0 2,400,592 - Dshi_2276 0.43 +0.2 2,400,602 - Dshi_2276 0.45 +0.4 2,400,645 + Dshi_2276 0.53 -2.2 2,400,647 + Dshi_2276 0.53 -1.3 2,400,647 + Dshi_2276 0.53 +0.9 2,400,647 + Dshi_2276 0.53 -1.6 2,400,663 - Dshi_2276 0.56 -1.7 2,400,668 - Dshi_2276 0.57 -0.6 2,400,669 + Dshi_2276 0.57 -0.4 2,400,724 - Dshi_2276 0.68 -1.2 2,400,728 + Dshi_2276 0.69 -0.8 2,400,732 + Dshi_2276 0.69 +0.3 2,400,732 + Dshi_2276 0.69 -1.5 2,400,733 + Dshi_2276 0.69 -1.4 2,400,740 - Dshi_2276 0.71 -1.1 2,400,750 + Dshi_2276 0.73 -1.7 2,400,778 - Dshi_2276 0.78 +0.1 2,400,783 - Dshi_2276 0.79 -0.2 2,400,783 - Dshi_2276 0.79 -1.8 2,400,795 + Dshi_2276 0.81 -3.0 2,400,810 + Dshi_2276 0.84 -1.9 2,400,810 + Dshi_2276 0.84 +0.6 2,400,810 + Dshi_2276 0.84 -1.6 2,400,818 - Dshi_2276 0.85 -0.1 2,400,920 - -2.1 2,400,927 + -0.4 2,400,927 + -0.9 2,400,927 + -0.9 2,400,950 + -0.7 2,400,950 + -2.6 2,400,951 - -1.7 2,401,040 + Dshi_2277 0.12 +1.4 2,401,040 + Dshi_2277 0.12 -1.4 2,401,116 + Dshi_2277 0.18 -1.0 2,401,139 - Dshi_2277 0.19 +0.0 2,401,150 - Dshi_2277 0.20 -0.8 2,401,182 - Dshi_2277 0.23 +1.2 2,401,239 - Dshi_2277 0.27 -0.9 2,401,304 - Dshi_2277 0.32 +0.5 2,401,308 + Dshi_2277 0.33 -1.4 2,401,308 + Dshi_2277 0.33 -0.2 2,401,311 - Dshi_2277 0.33 +0.4 2,401,316 - Dshi_2277 0.33 +1.8 2,401,334 + Dshi_2277 0.35 +4.5 2,401,347 + Dshi_2277 0.36 +1.2 2,401,347 + Dshi_2277 0.36 -0.7 2,401,406 + Dshi_2277 0.40 -0.3 2,401,472 + Dshi_2277 0.46 +0.4 2,401,472 + Dshi_2277 0.46 +1.2 2,401,473 - Dshi_2277 0.46 -0.3 2,401,497 + Dshi_2277 0.48 -1.3 2,401,535 - Dshi_2277 0.51 +0.6 2,401,537 + Dshi_2277 0.51 +0.0 2,401,538 + Dshi_2277 0.51 +0.0 2,401,603 + Dshi_2277 0.56 +1.0 2,401,613 + Dshi_2277 0.57 +3.2 2,401,627 + Dshi_2277 0.58 -1.1 2,401,687 + Dshi_2277 0.63 -0.8 2,401,688 - Dshi_2277 0.63 -1.1 2,401,747 + Dshi_2277 0.67 +0.2 2,401,914 + Dshi_2277 0.80 -0.9 2,401,922 - Dshi_2277 0.81 -2.9 2,401,929 + Dshi_2277 0.82 -0.2 2,401,957 - Dshi_2277 0.84 +1.0 2,401,995 + Dshi_2277 0.87 -1.2 2,402,009 + Dshi_2277 0.88 +0.2 2,402,029 - Dshi_2277 0.89 -1.4 2,402,086 + -1.8 2,402,092 - -1.5 2,402,094 - +1.2 2,402,139 - -1.1 2,402,231 + +0.3 2,402,243 + -0.5 2,402,252 - -1.5 2,402,291 - +0.6 2,402,339 + -2.1 2,402,352 - -2.1 2,402,395 - -0.4 2,402,433 + +0.2 2,402,441 - +0.9 2,402,464 + -0.9 2,402,486 + -1.3 2,402,488 - -1.8 2,402,494 - -0.2 2,402,550 + Dshi_2278 0.11 -0.8 2,402,593 + Dshi_2278 0.13 -0.7 2,402,601 - Dshi_2278 0.13 -1.4 2,402,601 - Dshi_2278 0.13 +4.0 2,402,624 - Dshi_2278 0.14 +0.1 2,402,660 + Dshi_2278 0.15 -0.1 2,402,660 + Dshi_2278 0.15 +0.0 2,402,660 + Dshi_2278 0.15 -0.9 2,402,665 + Dshi_2278 0.16 -1.8 2,402,668 - Dshi_2278 0.16 -0.2 2,402,668 - Dshi_2278 0.16 -2.4 2,402,668 - Dshi_2278 0.16 -0.8 2,402,678 - Dshi_2278 0.16 -0.6 2,402,691 - Dshi_2278 0.17 +0.2 2,402,712 + Dshi_2278 0.17 -1.6 2,402,838 + Dshi_2278 0.22 -1.0 2,402,857 + Dshi_2278 0.23 -0.8 2,402,864 - Dshi_2278 0.23 -1.0 2,402,894 + Dshi_2278 0.24 -1.0 2,402,894 + Dshi_2278 0.24 +2.4 2,402,924 + Dshi_2278 0.25 +0.7 2,402,928 + Dshi_2278 0.25 +0.1 2,402,936 - Dshi_2278 0.26 -1.6 2,402,961 - Dshi_2278 0.27 +1.2 2,403,082 + Dshi_2278 0.31 -0.8 2,403,091 - Dshi_2278 0.32 -0.9 2,403,130 + Dshi_2278 0.33 -0.1 2,403,140 + Dshi_2278 0.33 -1.5
Or see this region's nucleotide sequence