Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1867 and Dshi_1868 are separated by 61 nucleotides Dshi_1868 and Dshi_1869 are separated by 20 nucleotides Dshi_1869 and Dshi_1870 overlap by 1 nucleotides
Dshi_1867: Dshi_1867 - glutamine synthetase catalytic region (RefSeq), at 1,946,164 to 1,947,480
_1867
Dshi_1868: Dshi_1868 - ferredoxin-dependent glutamate synthase (RefSeq), at 1,947,542 to 1,948,876
_1868
Dshi_1869: Dshi_1869 - glutamate synthase alpha subunit domain protein (RefSeq), at 1,948,897 to 1,949,574
_1869
Dshi_1870: Dshi_1870 - glutamine amidotransferase class-II (RefSeq), at 1,949,574 to 1,950,500
_1870
Position (kb)
1947
1948
1949 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1946.647 kb on + strand, within Dshi_1867 at 1946.727 kb on - strand, within Dshi_1867 at 1946.771 kb on - strand, within Dshi_1867 at 1946.793 kb on + strand, within Dshi_1867 at 1946.955 kb on + strand, within Dshi_1867 at 1946.971 kb on - strand, within Dshi_1867 at 1946.976 kb on - strand, within Dshi_1867 at 1947.006 kb on + strand, within Dshi_1867 at 1947.192 kb on - strand, within Dshi_1867 at 1947.253 kb on - strand, within Dshi_1867 at 1947.377 kb on - strand at 1947.378 kb on - strand at 1947.380 kb on + strand at 1947.468 kb on + strand at 1947.468 kb on + strand at 1947.468 kb on + strand at 1947.549 kb on - strand at 1947.568 kb on + strand at 1947.577 kb on - strand at 1947.738 kb on + strand, within Dshi_1868 at 1947.773 kb on + strand, within Dshi_1868 at 1947.944 kb on + strand, within Dshi_1868 at 1947.957 kb on - strand, within Dshi_1868 at 1948.117 kb on - strand, within Dshi_1868 at 1948.137 kb on + strand, within Dshi_1868 at 1948.289 kb on - strand, within Dshi_1868 at 1948.430 kb on + strand, within Dshi_1868 at 1948.518 kb on - strand, within Dshi_1868 at 1948.553 kb on - strand, within Dshi_1868 at 1948.719 kb on + strand, within Dshi_1868 at 1948.784 kb on - strand at 1949.064 kb on - strand, within Dshi_1869 at 1949.139 kb on - strand, within Dshi_1869 at 1949.139 kb on - strand, within Dshi_1869 at 1949.141 kb on + strand at 1949.364 kb on + strand, within Dshi_1869 at 1949.388 kb on - strand, within Dshi_1869 at 1949.388 kb on - strand, within Dshi_1869 at 1949.481 kb on - strand, within Dshi_1869 at 1949.496 kb on + strand, within Dshi_1869 at 1949.556 kb on - strand at 1949.595 kb on + strand at 1949.603 kb on - strand at 1949.627 kb on - strand at 1949.672 kb on + strand, within Dshi_1870 at 1949.789 kb on - strand, within Dshi_1870 at 1949.830 kb on - strand, within Dshi_1870 at 1949.837 kb on - strand, within Dshi_1870
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 1,946,647 + Dshi_1867 0.37 -2.9 1,946,727 - Dshi_1867 0.43 -0.5 1,946,771 - Dshi_1867 0.46 +0.6 1,946,793 + Dshi_1867 0.48 -0.5 1,946,955 + Dshi_1867 0.60 -2.3 1,946,971 - Dshi_1867 0.61 -0.5 1,946,976 - Dshi_1867 0.62 -0.3 1,947,006 + Dshi_1867 0.64 +0.3 1,947,192 - Dshi_1867 0.78 -0.8 1,947,253 - Dshi_1867 0.83 +1.7 1,947,377 - -0.3 1,947,378 - -0.5 1,947,380 + -3.2 1,947,468 + -2.0 1,947,468 + -0.9 1,947,468 + +2.6 1,947,549 - +0.3 1,947,568 + -0.7 1,947,577 - -4.2 1,947,738 + Dshi_1868 0.15 +0.3 1,947,773 + Dshi_1868 0.17 +0.1 1,947,944 + Dshi_1868 0.30 -2.6 1,947,957 - Dshi_1868 0.31 -1.3 1,948,117 - Dshi_1868 0.43 +0.3 1,948,137 + Dshi_1868 0.45 -0.3 1,948,289 - Dshi_1868 0.56 -2.3 1,948,430 + Dshi_1868 0.67 +1.3 1,948,518 - Dshi_1868 0.73 -0.7 1,948,553 - Dshi_1868 0.76 +1.3 1,948,719 + Dshi_1868 0.88 -2.9 1,948,784 - +0.3 1,949,064 - Dshi_1869 0.25 +1.9 1,949,139 - Dshi_1869 0.36 +1.3 1,949,139 - Dshi_1869 0.36 +0.9 1,949,141 + +1.9 1,949,364 + Dshi_1869 0.69 -1.3 1,949,388 - Dshi_1869 0.72 +1.5 1,949,388 - Dshi_1869 0.72 +0.8 1,949,481 - Dshi_1869 0.86 -0.3 1,949,496 + Dshi_1869 0.88 -1.6 1,949,556 - +0.7 1,949,595 + -0.9 1,949,603 - -1.2 1,949,627 - -0.9 1,949,672 + Dshi_1870 0.11 +0.9 1,949,789 - Dshi_1870 0.23 +0.3 1,949,830 - Dshi_1870 0.28 -0.6 1,949,837 - Dshi_1870 0.28 +1.4
Or see this region's nucleotide sequence