Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1072 and Dshi_1073 are separated by 58 nucleotides Dshi_1073 and Dshi_1074 are separated by 31 nucleotides Dshi_1074 and Dshi_1075 are separated by 302 nucleotides Dshi_1075 and Dshi_1076 are separated by 175 nucleotides
Dshi_1072: Dshi_1072 - hypothetical protein (RefSeq), at 1,101,982 to 1,102,200
_1072
Dshi_1073: Dshi_1073 - Methionine synthase (RefSeq), at 1,102,259 to 1,103,038
_1073
Dshi_1074: Dshi_1074 - homocysteine S-methyltransferase (RefSeq), at 1,103,070 to 1,104,095
_1074
Dshi_1075: Dshi_1075 - protein of unknown function DUF1476 (RefSeq), at 1,104,398 to 1,104,712
_1075
Dshi_1076: Dshi_1076 - phosphoribosylaminoimidazole-succinocarboxamidesynthase (RefSeq), at 1,104,888 to 1,105,649
_1076
Position (kb)
1103
1104
1105 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1104.207 kb on - strand at 1104.542 kb on - strand, within Dshi_1075 at 1104.639 kb on + strand, within Dshi_1075 at 1104.710 kb on + strand at 1104.715 kb on - strand at 1104.743 kb on + strand at 1104.751 kb on - strand at 1104.751 kb on - strand at 1104.763 kb on + strand at 1104.769 kb on - strand at 1104.771 kb on - strand at 1104.829 kb on - strand at 1104.857 kb on + strand at 1104.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 1,104,207 - -1.7 1,104,542 - Dshi_1075 0.46 -0.6 1,104,639 + Dshi_1075 0.77 -2.1 1,104,710 + -2.6 1,104,715 - +0.4 1,104,743 + +0.1 1,104,751 - +0.4 1,104,751 - +0.4 1,104,763 + -0.1 1,104,769 - -0.0 1,104,771 - -0.4 1,104,829 - -4.4 1,104,857 + +1.4 1,104,870 - +0.2
Or see this region's nucleotide sequence