Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1046 and Dshi_1047 overlap by 4 nucleotides Dshi_1047 and Dshi_1048 are separated by 1 nucleotides Dshi_1048 and Dshi_1049 overlap by 4 nucleotides Dshi_1049 and Dshi_1050 are separated by 7 nucleotides
Dshi_1046: Dshi_1046 - Amidase (RefSeq), at 1,079,893 to 1,081,023
_1046
Dshi_1047: Dshi_1047 - hypothetical protein (RefSeq), at 1,081,020 to 1,081,880
_1047
Dshi_1048: Dshi_1048 - Enoyl-CoA hydratase/isomerase (RefSeq), at 1,081,882 to 1,082,679
_1048
Dshi_1049: Dshi_1049 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,082,676 to 1,083,461
_1049
Dshi_1050: Dshi_1050 - hypothetical protein (RefSeq), at 1,083,469 to 1,083,843
_1050
Position (kb)
1081
1082
1083 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1080.895 kb on + strand, within Dshi_1046 at 1080.936 kb on + strand at 1080.946 kb on + strand at 1080.965 kb on - strand at 1081.239 kb on - strand, within Dshi_1047 at 1081.244 kb on + strand, within Dshi_1047 at 1081.252 kb on - strand, within Dshi_1047 at 1081.254 kb on + strand, within Dshi_1047 at 1081.262 kb on - strand, within Dshi_1047 at 1081.269 kb on + strand, within Dshi_1047 at 1081.269 kb on + strand, within Dshi_1047 at 1081.345 kb on + strand, within Dshi_1047 at 1081.348 kb on - strand, within Dshi_1047 at 1081.358 kb on - strand, within Dshi_1047 at 1081.454 kb on + strand, within Dshi_1047 at 1081.454 kb on + strand, within Dshi_1047 at 1081.458 kb on - strand, within Dshi_1047 at 1081.462 kb on - strand, within Dshi_1047 at 1081.564 kb on - strand, within Dshi_1047 at 1081.658 kb on + strand, within Dshi_1047 at 1081.658 kb on + strand, within Dshi_1047 at 1081.663 kb on + strand, within Dshi_1047 at 1081.782 kb on - strand, within Dshi_1047 at 1081.960 kb on + strand at 1082.055 kb on - strand, within Dshi_1048 at 1082.182 kb on - strand, within Dshi_1048 at 1082.424 kb on - strand, within Dshi_1048 at 1082.424 kb on - strand, within Dshi_1048 at 1082.608 kb on + strand at 1082.672 kb on + strand at 1082.769 kb on - strand, within Dshi_1049 at 1082.836 kb on - strand, within Dshi_1049 at 1082.860 kb on + strand, within Dshi_1049 at 1082.872 kb on + strand, within Dshi_1049 at 1082.874 kb on - strand, within Dshi_1049 at 1082.931 kb on - strand, within Dshi_1049 at 1082.936 kb on + strand, within Dshi_1049 at 1083.009 kb on + strand, within Dshi_1049 at 1083.062 kb on + strand, within Dshi_1049 at 1083.118 kb on - strand, within Dshi_1049 at 1083.357 kb on + strand, within Dshi_1049 at 1083.466 kb on - strand at 1083.475 kb on - strand at 1083.486 kb on - strand at 1083.646 kb on + strand, within Dshi_1050
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 1,080,895 + Dshi_1046 0.89 +2.9 1,080,936 + -1.3 1,080,946 + +1.3 1,080,965 - -2.7 1,081,239 - Dshi_1047 0.25 +0.3 1,081,244 + Dshi_1047 0.26 +0.3 1,081,252 - Dshi_1047 0.27 -2.7 1,081,254 + Dshi_1047 0.27 -0.3 1,081,262 - Dshi_1047 0.28 +1.3 1,081,269 + Dshi_1047 0.29 -2.0 1,081,269 + Dshi_1047 0.29 -2.0 1,081,345 + Dshi_1047 0.38 +0.5 1,081,348 - Dshi_1047 0.38 +0.3 1,081,358 - Dshi_1047 0.39 +0.9 1,081,454 + Dshi_1047 0.50 +0.3 1,081,454 + Dshi_1047 0.50 +1.3 1,081,458 - Dshi_1047 0.51 -2.5 1,081,462 - Dshi_1047 0.51 -0.7 1,081,564 - Dshi_1047 0.63 +0.3 1,081,658 + Dshi_1047 0.74 +1.9 1,081,658 + Dshi_1047 0.74 +2.3 1,081,663 + Dshi_1047 0.75 -1.3 1,081,782 - Dshi_1047 0.89 -3.1 1,081,960 + +0.3 1,082,055 - Dshi_1048 0.22 -1.7 1,082,182 - Dshi_1048 0.38 +1.3 1,082,424 - Dshi_1048 0.68 -1.8 1,082,424 - Dshi_1048 0.68 -1.3 1,082,608 + -0.7 1,082,672 + -4.0 1,082,769 - Dshi_1049 0.12 -2.9 1,082,836 - Dshi_1049 0.20 +0.3 1,082,860 + Dshi_1049 0.23 +1.9 1,082,872 + Dshi_1049 0.25 -1.3 1,082,874 - Dshi_1049 0.25 +0.9 1,082,931 - Dshi_1049 0.32 -1.5 1,082,936 + Dshi_1049 0.33 -0.7 1,083,009 + Dshi_1049 0.42 -0.1 1,083,062 + Dshi_1049 0.49 -1.4 1,083,118 - Dshi_1049 0.56 -2.3 1,083,357 + Dshi_1049 0.87 -0.7 1,083,466 - +0.3 1,083,475 - +0.3 1,083,486 - -2.3 1,083,646 + Dshi_1050 0.47 -2.0
Or see this region's nucleotide sequence