Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0825 and Dshi_0826 are separated by 180 nucleotides Dshi_0826 and Dshi_0827 are separated by 173 nucleotides Dshi_0827 and Dshi_0828 overlap by 4 nucleotides
Dshi_0825: Dshi_0825 - AMP-dependent synthetase and ligase (RefSeq), at 830,837 to 832,729
_0825
Dshi_0826: Dshi_0826 - malic protein NAD-binding (RefSeq), at 832,910 to 835,189
_0826
Dshi_0827: Dshi_0827 - cytidine deaminase (RefSeq), at 835,363 to 835,755
_0827
Dshi_0828: Dshi_0828 - pyrimidine-nucleoside phosphorylase (RefSeq), at 835,752 to 837,059
_0828
Position (kb) 832
833
834
835
836 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 831.916 kb on + strand, within Dshi_0825 at 831.917 kb on - strand, within Dshi_0825 at 831.950 kb on - strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.987 kb on + strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.995 kb on - strand, within Dshi_0825 at 831.997 kb on + strand, within Dshi_0825 at 832.022 kb on - strand, within Dshi_0825 at 832.046 kb on - strand, within Dshi_0825 at 832.145 kb on + strand, within Dshi_0825 at 832.193 kb on + strand, within Dshi_0825 at 832.213 kb on - strand, within Dshi_0825 at 832.579 kb on - strand at 832.614 kb on + strand at 832.707 kb on + strand at 832.707 kb on + strand at 832.715 kb on - strand at 832.715 kb on - strand at 832.715 kb on - strand at 832.717 kb on + strand at 832.765 kb on + strand at 832.949 kb on + strand at 832.993 kb on - strand at 833.070 kb on + strand at 833.152 kb on + strand, within Dshi_0826 at 833.188 kb on - strand, within Dshi_0826 at 833.233 kb on - strand, within Dshi_0826 at 833.274 kb on - strand, within Dshi_0826 at 833.291 kb on - strand, within Dshi_0826 at 833.313 kb on - strand, within Dshi_0826 at 833.383 kb on + strand, within Dshi_0826 at 833.386 kb on + strand, within Dshi_0826 at 833.457 kb on - strand, within Dshi_0826 at 833.658 kb on + strand, within Dshi_0826 at 833.775 kb on - strand, within Dshi_0826 at 833.776 kb on + strand, within Dshi_0826 at 833.921 kb on - strand, within Dshi_0826 at 834.124 kb on - strand, within Dshi_0826 at 834.268 kb on + strand, within Dshi_0826 at 834.300 kb on - strand, within Dshi_0826 at 834.323 kb on - strand, within Dshi_0826 at 834.449 kb on + strand, within Dshi_0826 at 834.734 kb on - strand, within Dshi_0826 at 834.767 kb on - strand, within Dshi_0826 at 834.817 kb on - strand, within Dshi_0826 at 834.817 kb on - strand, within Dshi_0826 at 834.889 kb on + strand, within Dshi_0826 at 834.902 kb on - strand, within Dshi_0826 at 834.921 kb on - strand, within Dshi_0826 at 834.999 kb on - strand at 835.111 kb on + strand at 835.111 kb on + strand at 835.344 kb on - strand at 835.379 kb on - strand at 835.418 kb on - strand, within Dshi_0827 at 835.459 kb on - strand, within Dshi_0827 at 835.469 kb on - strand, within Dshi_0827 at 835.597 kb on - strand, within Dshi_0827 at 835.622 kb on + strand, within Dshi_0827 at 836.098 kb on - strand, within Dshi_0828
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 831,916 + Dshi_0825 0.57 +1.9 831,917 - Dshi_0825 0.57 +0.2 831,950 - Dshi_0825 0.59 -0.5 831,987 + Dshi_0825 0.61 -2.1 831,987 + Dshi_0825 0.61 +0.2 831,987 + Dshi_0825 0.61 -2.3 831,987 + Dshi_0825 0.61 +0.3 831,995 - Dshi_0825 0.61 -1.1 831,995 - Dshi_0825 0.61 -0.2 831,995 - Dshi_0825 0.61 +0.5 831,995 - Dshi_0825 0.61 -0.3 831,995 - Dshi_0825 0.61 -0.3 831,997 + Dshi_0825 0.61 +0.5 832,022 - Dshi_0825 0.63 +0.1 832,046 - Dshi_0825 0.64 -0.4 832,145 + Dshi_0825 0.69 +0.5 832,193 + Dshi_0825 0.72 -1.5 832,213 - Dshi_0825 0.73 -1.5 832,579 - -1.2 832,614 + -0.4 832,707 + -0.7 832,707 + +0.2 832,715 - -0.1 832,715 - -1.0 832,715 - +0.6 832,717 + +0.0 832,765 + +0.1 832,949 + +0.5 832,993 - -0.7 833,070 + -2.1 833,152 + Dshi_0826 0.11 -3.0 833,188 - Dshi_0826 0.12 -1.9 833,233 - Dshi_0826 0.14 -4.8 833,274 - Dshi_0826 0.16 +0.5 833,291 - Dshi_0826 0.17 +1.5 833,313 - Dshi_0826 0.18 -2.0 833,383 + Dshi_0826 0.21 -1.5 833,386 + Dshi_0826 0.21 -2.1 833,457 - Dshi_0826 0.24 -0.8 833,658 + Dshi_0826 0.33 +0.5 833,775 - Dshi_0826 0.38 -2.0 833,776 + Dshi_0826 0.38 -0.1 833,921 - Dshi_0826 0.44 -1.5 834,124 - Dshi_0826 0.53 -2.2 834,268 + Dshi_0826 0.60 -2.7 834,300 - Dshi_0826 0.61 +0.5 834,323 - Dshi_0826 0.62 +0.3 834,449 + Dshi_0826 0.68 -3.4 834,734 - Dshi_0826 0.80 -1.1 834,767 - Dshi_0826 0.81 -3.6 834,817 - Dshi_0826 0.84 -1.1 834,817 - Dshi_0826 0.84 -3.3 834,889 + Dshi_0826 0.87 -3.6 834,902 - Dshi_0826 0.87 -3.7 834,921 - Dshi_0826 0.88 -1.1 834,999 - -1.3 835,111 + -2.7 835,111 + -3.1 835,344 - +2.8 835,379 - +0.8 835,418 - Dshi_0827 0.14 +2.1 835,459 - Dshi_0827 0.24 +0.5 835,469 - Dshi_0827 0.27 -0.6 835,597 - Dshi_0827 0.60 -0.5 835,622 + Dshi_0827 0.66 -3.3 836,098 - Dshi_0828 0.26 -1.2
Or see this region's nucleotide sequence