Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0718 and Dshi_0719 are separated by 99 nucleotides Dshi_0719 and Dshi_0720 overlap by 8 nucleotides Dshi_0720 and Dshi_0721 overlap by 4 nucleotides Dshi_0721 and Dshi_0722 are separated by 14 nucleotides
Dshi_0718: Dshi_0718 - Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) (from data) , at 726,256 to 727,788
_0718
Dshi_0719: Dshi_0719 - hypothetical protein (RefSeq), at 727,888 to 728,286
_0719
Dshi_0720: Dshi_0720 - Endoribonuclease L-PSP (RefSeq), at 728,279 to 728,668
_0720
Dshi_0721: Dshi_0721 - hypothetical protein (RefSeq), at 728,665 to 729,036
_0721
Dshi_0722: Dshi_0722 - hypothetical protein (RefSeq), at 729,051 to 729,410
_0722
Position (kb)
727
728
729 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 727.210 kb on - strand, within Dshi_0718 at 727.230 kb on + strand, within Dshi_0718 at 727.257 kb on - strand, within Dshi_0718 at 727.355 kb on + strand, within Dshi_0718 at 727.428 kb on + strand, within Dshi_0718 at 727.428 kb on + strand, within Dshi_0718 at 727.441 kb on + strand, within Dshi_0718 at 727.456 kb on + strand, within Dshi_0718 at 727.457 kb on - strand, within Dshi_0718 at 727.467 kb on + strand, within Dshi_0718 at 727.510 kb on + strand, within Dshi_0718 at 727.590 kb on - strand, within Dshi_0718 at 727.836 kb on - strand at 727.875 kb on - strand at 727.880 kb on - strand at 727.915 kb on + strand at 727.923 kb on + strand at 728.062 kb on + strand, within Dshi_0719 at 728.226 kb on + strand, within Dshi_0719 at 728.236 kb on + strand, within Dshi_0719 at 728.409 kb on + strand, within Dshi_0720 at 728.440 kb on + strand, within Dshi_0720 at 728.562 kb on - strand, within Dshi_0720 at 728.562 kb on - strand, within Dshi_0720 at 728.580 kb on - strand, within Dshi_0720 at 728.643 kb on - strand at 728.890 kb on + strand, within Dshi_0721 at 729.192 kb on + strand, within Dshi_0722 at 729.192 kb on + strand, within Dshi_0722
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 727,210 - Dshi_0718 0.62 +3.0 727,230 + Dshi_0718 0.64 +2.4 727,257 - Dshi_0718 0.65 +0.1 727,355 + Dshi_0718 0.72 +0.4 727,428 + Dshi_0718 0.76 -2.2 727,428 + Dshi_0718 0.76 +1.4 727,441 + Dshi_0718 0.77 +0.4 727,456 + Dshi_0718 0.78 -2.1 727,457 - Dshi_0718 0.78 -1.0 727,467 + Dshi_0718 0.79 +1.4 727,510 + Dshi_0718 0.82 -1.3 727,590 - Dshi_0718 0.87 -1.3 727,836 - +0.2 727,875 - +1.1 727,880 - +0.6 727,915 + +0.2 727,923 + +0.4 728,062 + Dshi_0719 0.44 +0.4 728,226 + Dshi_0719 0.85 -2.6 728,236 + Dshi_0719 0.87 -1.2 728,409 + Dshi_0720 0.33 -2.6 728,440 + Dshi_0720 0.41 +0.4 728,562 - Dshi_0720 0.73 -1.2 728,562 - Dshi_0720 0.73 -3.9 728,580 - Dshi_0720 0.77 +0.1 728,643 - -0.2 728,890 + Dshi_0721 0.60 +0.1 729,192 + Dshi_0722 0.39 -2.4 729,192 + Dshi_0722 0.39 -1.6
Or see this region's nucleotide sequence