Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0320 and Dshi_0321 are separated by 15 nucleotides Dshi_0321 and Dshi_0322 are separated by 43 nucleotides Dshi_0322 and Dshi_0323 overlap by 4 nucleotides Dshi_0323 and Dshi_0324 are separated by 52 nucleotides
Dshi_0320: Dshi_0320 - polar amino acid ABC transporter, inner membrane subunit (RefSeq), at 308,666 to 309,964
_0320
Dshi_0321: Dshi_0321 - ABC transporter related (RefSeq), at 309,980 to 310,756
_0321
Dshi_0322: Dshi_0322 - putative phosphohistidine phosphatase, SixA (RefSeq), at 310,800 to 311,300
_0322
Dshi_0323: Dshi_0323 - hypothetical protein (RefSeq), at 311,297 to 311,920
_0323
Dshi_0324: Dshi_0324 - acetylglutamate kinase (RefSeq), at 311,973 to 312,836
_0324
Position (kb) 310
311
312 Strain fitness (log2 ratio) -2
-1
0
1 at 309.858 kb on + strand at 309.930 kb on + strand at 309.938 kb on - strand at 310.000 kb on - strand at 310.014 kb on + strand at 310.101 kb on + strand, within Dshi_0321 at 310.156 kb on + strand, within Dshi_0321 at 310.254 kb on + strand, within Dshi_0321 at 310.254 kb on + strand, within Dshi_0321 at 310.257 kb on - strand, within Dshi_0321 at 310.548 kb on - strand, within Dshi_0321 at 311.516 kb on + strand, within Dshi_0323 at 311.523 kb on + strand, within Dshi_0323 at 311.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 309,858 + -0.2 309,930 + -2.1 309,938 - -2.2 310,000 - +0.7 310,014 + -1.2 310,101 + Dshi_0321 0.16 -1.6 310,156 + Dshi_0321 0.23 +0.5 310,254 + Dshi_0321 0.35 -0.3 310,254 + Dshi_0321 0.35 +0.1 310,257 - Dshi_0321 0.36 +0.2 310,548 - Dshi_0321 0.73 -0.4 311,516 + Dshi_0323 0.35 +0.4 311,523 + Dshi_0323 0.36 -2.6 311,977 - +0.8
Or see this region's nucleotide sequence