Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0194 and Dshi_0195 are separated by 37 nucleotides Dshi_0195 and Dshi_0196 are separated by 25 nucleotides Dshi_0196 and Dshi_0197 overlap by 4 nucleotides Dshi_0197 and Dshi_0198 are separated by 139 nucleotides
Dshi_0194: Dshi_0194 - (p)ppGpp synthetase I, SpoT/RelA (RefSeq), at 193,417 to 195,561
_0194
Dshi_0195: Dshi_0195 - hypothetical protein (RefSeq), at 195,599 to 196,255
_0195
Dshi_0196: Dshi_0196 - pyridoxal phosphate biosynthetic protein PdxJ (RefSeq), at 196,281 to 197,045
_0196
Dshi_0197: Dshi_0197 - hypothetical protein (RefSeq), at 197,042 to 197,704
_0197
Dshi_0198: Dshi_0198 - holo-acyl-carrier-protein synthase (RefSeq), at 197,844 to 198,263
_0198
Position (kb) 196
197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 195.290 kb on + strand, within Dshi_0194 at 195.316 kb on + strand, within Dshi_0194 at 195.454 kb on + strand at 195.460 kb on - strand at 195.475 kb on - strand at 195.475 kb on - strand at 195.549 kb on - strand at 195.554 kb on + strand at 195.562 kb on - strand at 195.666 kb on - strand, within Dshi_0195 at 195.666 kb on - strand, within Dshi_0195 at 195.678 kb on + strand, within Dshi_0195 at 195.887 kb on - strand, within Dshi_0195 at 195.887 kb on - strand, within Dshi_0195 at 195.904 kb on + strand, within Dshi_0195 at 195.912 kb on - strand, within Dshi_0195 at 195.957 kb on + strand, within Dshi_0195 at 195.963 kb on - strand, within Dshi_0195 at 195.972 kb on + strand, within Dshi_0195 at 195.990 kb on - strand, within Dshi_0195 at 195.995 kb on + strand, within Dshi_0195 at 196.072 kb on + strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.080 kb on - strand, within Dshi_0195 at 196.142 kb on - strand, within Dshi_0195 at 196.171 kb on + strand, within Dshi_0195 at 196.295 kb on - strand at 197.024 kb on + strand at 197.039 kb on + strand at 197.057 kb on - strand at 197.092 kb on - strand at 197.578 kb on - strand, within Dshi_0197 at 197.578 kb on - strand, within Dshi_0197 at 197.588 kb on + strand, within Dshi_0197 at 197.595 kb on + strand, within Dshi_0197 at 197.694 kb on - strand at 197.716 kb on + strand at 197.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 195,290 + Dshi_0194 0.87 +2.4 195,316 + Dshi_0194 0.89 -0.6 195,454 + -0.6 195,460 - +0.5 195,475 - -0.2 195,475 - -2.7 195,549 - -0.3 195,554 + -0.6 195,562 - +0.4 195,666 - Dshi_0195 0.10 +0.4 195,666 - Dshi_0195 0.10 +1.4 195,678 + Dshi_0195 0.12 +1.1 195,887 - Dshi_0195 0.44 +2.0 195,887 - Dshi_0195 0.44 +1.2 195,904 + Dshi_0195 0.46 -2.2 195,912 - Dshi_0195 0.48 -0.1 195,957 + Dshi_0195 0.54 +1.0 195,963 - Dshi_0195 0.55 -4.2 195,972 + Dshi_0195 0.57 +2.0 195,990 - Dshi_0195 0.60 -0.9 195,995 + Dshi_0195 0.60 -1.0 196,072 + Dshi_0195 0.72 -1.9 196,080 - Dshi_0195 0.73 -0.9 196,080 - Dshi_0195 0.73 +1.2 196,080 - Dshi_0195 0.73 +4.0 196,142 - Dshi_0195 0.83 -0.5 196,171 + Dshi_0195 0.87 -1.7 196,295 - +0.0 197,024 + -1.9 197,039 + -1.9 197,057 - -0.9 197,092 - -0.6 197,578 - Dshi_0197 0.81 +0.4 197,578 - Dshi_0197 0.81 +0.6 197,588 + Dshi_0197 0.82 -0.6 197,595 + Dshi_0197 0.83 +0.3 197,694 - +0.4 197,716 + +0.4 197,821 - -1.1
Or see this region's nucleotide sequence