Strain Fitness in Dinoroseobacter shibae DFL-12 around Dshi_0026

Experiment: m.b. Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDshi_0025 and Dshi_0026 are separated by 73 nucleotidesDshi_0026 and Dshi_0027 are separated by 123 nucleotides Dshi_0025: Dshi_0025 - anti-ECFsigma factor, ChrR (RefSeq), at 29,143 to 29,814 _0025 Dshi_0026: Dshi_0026 - NAD-dependent epimerase/dehydratase (RefSeq), at 29,888 to 30,529 _0026 Dshi_0027: Dshi_0027 - Alkane 1-monooxygenase (RefSeq), at 30,653 to 31,807 _0027 Position (kb) 29 30 31Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 28.889 kb on + strandat 28.945 kb on - strandat 28.964 kb on - strandat 29.006 kb on + strandat 29.006 kb on + strandat 29.190 kb on + strandat 29.191 kb on + strandat 29.198 kb on - strandat 29.337 kb on - strand, within Dshi_0025at 29.390 kb on + strand, within Dshi_0025at 29.398 kb on - strand, within Dshi_0025at 29.434 kb on + strand, within Dshi_0025at 29.436 kb on - strand, within Dshi_0025at 29.463 kb on - strand, within Dshi_0025at 29.469 kb on + strand, within Dshi_0025at 29.575 kb on + strand, within Dshi_0025at 29.586 kb on + strand, within Dshi_0025at 29.667 kb on + strand, within Dshi_0025at 29.723 kb on + strand, within Dshi_0025at 29.731 kb on - strandat 29.821 kb on - strandat 29.822 kb on - strandat 29.838 kb on - strandat 29.996 kb on + strand, within Dshi_0026at 30.039 kb on - strand, within Dshi_0026at 30.258 kb on - strand, within Dshi_0026at 30.314 kb on + strand, within Dshi_0026at 30.337 kb on - strand, within Dshi_0026at 30.341 kb on + strand, within Dshi_0026at 30.341 kb on + strand, within Dshi_0026at 30.351 kb on + strand, within Dshi_0026at 30.395 kb on + strand, within Dshi_0026at 30.403 kb on - strand, within Dshi_0026at 30.419 kb on + strand, within Dshi_0026at 30.703 kb on - strandat 30.756 kb on + strandat 30.780 kb on + strand, within Dshi_0027at 30.811 kb on + strand, within Dshi_0027at 30.821 kb on + strand, within Dshi_0027at 30.836 kb on + strand, within Dshi_0027at 30.837 kb on + strand, within Dshi_0027at 30.857 kb on - strand, within Dshi_0027at 30.920 kb on + strand, within Dshi_0027at 30.982 kb on - strand, within Dshi_0027at 30.982 kb on - strand, within Dshi_0027at 31.019 kb on - strand, within Dshi_0027at 31.102 kb on - strand, within Dshi_0027at 31.145 kb on + strand, within Dshi_0027at 31.192 kb on + strand, within Dshi_0027at 31.197 kb on + strand, within Dshi_0027at 31.234 kb on + strand, within Dshi_0027at 31.308 kb on + strand, within Dshi_0027at 31.335 kb on + strand, within Dshi_0027at 31.343 kb on - strand, within Dshi_0027at 31.350 kb on + strand, within Dshi_0027at 31.377 kb on - strand, within Dshi_0027

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Per-strain Table

Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM
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28,889 + -0.6
28,945 - +0.4
28,964 - -2.1
29,006 + +2.4
29,006 + +0.4
29,190 + -0.4
29,191 + -0.1
29,198 - -0.4
29,337 - Dshi_0025 0.29 +0.0
29,390 + Dshi_0025 0.37 -0.8
29,398 - Dshi_0025 0.38 +0.2
29,434 + Dshi_0025 0.43 -0.4
29,436 - Dshi_0025 0.44 +2.0
29,463 - Dshi_0025 0.48 +0.4
29,469 + Dshi_0025 0.49 -0.9
29,575 + Dshi_0025 0.64 -2.1
29,586 + Dshi_0025 0.66 +0.0
29,667 + Dshi_0025 0.78 -1.1
29,723 + Dshi_0025 0.86 -2.4
29,731 - +0.4
29,821 - +1.6
29,822 - -1.1
29,838 - -0.6
29,996 + Dshi_0026 0.17 +0.6
30,039 - Dshi_0026 0.24 +0.4
30,258 - Dshi_0026 0.58 +0.0
30,314 + Dshi_0026 0.66 +1.4
30,337 - Dshi_0026 0.70 -0.1
30,341 + Dshi_0026 0.71 -0.3
30,341 + Dshi_0026 0.71 -2.4
30,351 + Dshi_0026 0.72 +1.4
30,395 + Dshi_0026 0.79 -0.4
30,403 - Dshi_0026 0.80 -0.3
30,419 + Dshi_0026 0.83 -0.2
30,703 - +0.7
30,756 + +0.4
30,780 + Dshi_0027 0.11 -2.6
30,811 + Dshi_0027 0.14 -0.3
30,821 + Dshi_0027 0.15 +0.4
30,836 + Dshi_0027 0.16 -0.1
30,837 + Dshi_0027 0.16 -1.1
30,857 - Dshi_0027 0.18 -1.6
30,920 + Dshi_0027 0.23 +0.4
30,982 - Dshi_0027 0.28 -1.6
30,982 - Dshi_0027 0.28 -1.7
31,019 - Dshi_0027 0.32 +0.4
31,102 - Dshi_0027 0.39 +0.4
31,145 + Dshi_0027 0.43 -2.1
31,192 + Dshi_0027 0.47 +0.4
31,197 + Dshi_0027 0.47 -3.1
31,234 + Dshi_0027 0.50 +0.4
31,308 + Dshi_0027 0.57 -1.1
31,335 + Dshi_0027 0.59 +1.3
31,343 - Dshi_0027 0.60 +0.4
31,350 + Dshi_0027 0.60 -1.5
31,377 - Dshi_0027 0.63 -1.9

Or see this region's nucleotide sequence