Experiment: copper (II) chloride 0.32 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmE and ccmD overlap by 4 nucleotides ccmD and ccmC overlap by 1 nucleotides ccmC and ccmB are separated by 1 nucleotides ccmB and ccmA are separated by 11 nucleotides
SO0259: ccmE - cytochrome c biogenesis protein CcmE (NCBI ptt file), at 260,541 to 261,026
ccmE
SO0260: ccmD - heme exporter protein CcmD (NCBI ptt file), at 261,023 to 261,223
ccmD
SO0261: ccmC - heme exporter protein CcmC (NCBI ptt file), at 261,223 to 261,969
ccmC
SO0262: ccmB - heme exporter protein CcmB (NCBI ptt file), at 261,971 to 262,657
ccmB
SO0263: ccmA - heme exporter protein CcmA (NCBI ptt file), at 262,669 to 263,319
ccmA
Position (kb) 261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.430 kb on + strand at 260.471 kb on - strand at 260.646 kb on + strand, within ccmE at 260.821 kb on - strand, within ccmE at 260.858 kb on - strand, within ccmE at 261.000 kb on - strand at 261.581 kb on - strand, within ccmC at 261.600 kb on + strand, within ccmC at 261.638 kb on + strand, within ccmC at 261.801 kb on - strand, within ccmC at 261.952 kb on - strand at 262.012 kb on + strand at 262.226 kb on + strand, within ccmB at 262.372 kb on + strand, within ccmB at 262.377 kb on + strand, within ccmB at 262.401 kb on - strand, within ccmB at 262.641 kb on - strand at 262.666 kb on + strand at 262.668 kb on - strand at 262.876 kb on - strand, within ccmA at 262.899 kb on - strand, within ccmA
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 0.32 mM remove 260,430 + +0.1 260,471 - +0.6 260,646 + ccmE SO0259 0.22 -0.3 260,821 - ccmE SO0259 0.58 +0.3 260,858 - ccmE SO0259 0.65 +0.0 261,000 - -0.8 261,581 - ccmC SO0261 0.48 -1.2 261,600 + ccmC SO0261 0.50 -0.5 261,638 + ccmC SO0261 0.56 -0.4 261,801 - ccmC SO0261 0.77 -0.6 261,952 - -1.0 262,012 + -1.3 262,226 + ccmB SO0262 0.37 -1.2 262,372 + ccmB SO0262 0.58 -1.6 262,377 + ccmB SO0262 0.59 -3.4 262,401 - ccmB SO0262 0.63 -1.2 262,641 - -1.0 262,666 + -1.4 262,668 - +0.2 262,876 - ccmA SO0263 0.32 -2.7 262,899 - ccmA SO0263 0.35 -0.6
Or see this region's nucleotide sequence