Strain Fitness in Shewanella oneidensis MR-1 around SO0174

Experiment: copper (II) chloride 0.32 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspK and gspL are separated by 48 nucleotidesgspL and gspM are separated by 1 nucleotidesgspM and gspN are separated by 14 nucleotides SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385 gspK SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621 gspL SO0175: gspM - general secretion pathway protein M (NCBI ptt file), at 181,623 to 182,099 gspM SO0176: gspN - general secretion pathway protein N (NCBI ptt file), at 182,114 to 182,875 gspN Position (kb) 180 181 182Strain fitness (log2 ratio) -2 -1 0 1at 179.436 kb on - strandat 179.544 kb on - strand, within gspKat 179.635 kb on + strand, within gspKat 179.645 kb on + strand, within gspKat 179.785 kb on + strand, within gspKat 179.849 kb on - strand, within gspKat 179.892 kb on + strand, within gspKat 180.055 kb on + strand, within gspKat 180.102 kb on - strand, within gspKat 180.209 kb on - strand, within gspKat 180.410 kb on + strandat 180.418 kb on - strandat 180.424 kb on + strandat 180.476 kb on - strandat 180.493 kb on + strandat 180.538 kb on + strandat 180.546 kb on - strandat 180.546 kb on - strandat 180.569 kb on - strand, within gspLat 180.569 kb on - strand, within gspLat 180.569 kb on - strand, within gspLat 180.628 kb on - strand, within gspLat 180.628 kb on - strand, within gspLat 180.697 kb on - strand, within gspLat 180.910 kb on - strand, within gspLat 180.920 kb on + strand, within gspLat 180.932 kb on + strand, within gspLat 181.035 kb on + strand, within gspLat 181.078 kb on - strand, within gspLat 181.147 kb on - strand, within gspLat 181.225 kb on - strand, within gspLat 181.281 kb on - strand, within gspLat 181.311 kb on - strand, within gspLat 181.364 kb on + strand, within gspLat 181.364 kb on + strand, within gspLat 181.372 kb on - strand, within gspLat 181.372 kb on - strand, within gspLat 181.404 kb on - strand, within gspLat 181.416 kb on + strand, within gspLat 181.591 kb on - strandat 181.604 kb on + strandat 181.612 kb on - strandat 181.651 kb on + strandat 181.659 kb on - strandat 181.659 kb on - strandat 181.659 kb on - strandat 181.734 kb on + strand, within gspMat 181.754 kb on - strand, within gspMat 181.798 kb on + strand, within gspMat 181.881 kb on - strand, within gspMat 182.067 kb on + strandat 182.081 kb on + strandat 182.104 kb on + strandat 182.149 kb on + strandat 182.149 kb on + strandat 182.152 kb on - strandat 182.171 kb on + strandat 182.229 kb on + strand, within gspNat 182.319 kb on + strand, within gspNat 182.404 kb on - strand, within gspNat 182.420 kb on + strand, within gspNat 182.425 kb on - strand, within gspNat 182.548 kb on + strand, within gspN

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 0.32 mM
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179,436 - -0.7
179,544 - gspK SO0173 0.16 -1.1
179,635 + gspK SO0173 0.25 -0.3
179,645 + gspK SO0173 0.26 -0.1
179,785 + gspK SO0173 0.40 -0.1
179,849 - gspK SO0173 0.46 -1.5
179,892 + gspK SO0173 0.51 -0.2
180,055 + gspK SO0173 0.67 -0.7
180,102 - gspK SO0173 0.72 -0.1
180,209 - gspK SO0173 0.82 -0.8
180,410 + -0.3
180,418 - -0.3
180,424 + -0.3
180,476 - -1.5
180,493 + -1.5
180,538 + -1.5
180,546 - -0.8
180,546 - -1.1
180,569 - gspL SO0174 0.11 -1.5
180,569 - gspL SO0174 0.11 -0.1
180,569 - gspL SO0174 0.11 -2.4
180,628 - gspL SO0174 0.16 -0.4
180,628 - gspL SO0174 0.16 -1.2
180,697 - gspL SO0174 0.22 -1.4
180,910 - gspL SO0174 0.40 -0.8
180,920 + gspL SO0174 0.41 -0.7
180,932 + gspL SO0174 0.42 -0.8
181,035 + gspL SO0174 0.51 -0.5
181,078 - gspL SO0174 0.54 -1.5
181,147 - gspL SO0174 0.60 -0.1
181,225 - gspL SO0174 0.67 -2.7
181,281 - gspL SO0174 0.71 -1.9
181,311 - gspL SO0174 0.74 -1.4
181,364 + gspL SO0174 0.78 -0.8
181,364 + gspL SO0174 0.78 +0.2
181,372 - gspL SO0174 0.79 -2.1
181,372 - gspL SO0174 0.79 -1.8
181,404 - gspL SO0174 0.82 -1.0
181,416 + gspL SO0174 0.83 -1.1
181,591 - -0.2
181,604 + -0.1
181,612 - -2.2
181,651 + -0.8
181,659 - -0.9
181,659 - -0.9
181,659 - -1.3
181,734 + gspM SO0175 0.23 -1.4
181,754 - gspM SO0175 0.27 -1.7
181,798 + gspM SO0175 0.37 -1.4
181,881 - gspM SO0175 0.54 -0.4
182,067 + -0.2
182,081 + -0.2
182,104 + -0.4
182,149 + -0.9
182,149 + -0.6
182,152 - -0.5
182,171 + -2.1
182,229 + gspN SO0176 0.15 -0.6
182,319 + gspN SO0176 0.27 -0.2
182,404 - gspN SO0176 0.38 -0.9
182,420 + gspN SO0176 0.40 -2.0
182,425 - gspN SO0176 0.41 -0.7
182,548 + gspN SO0176 0.57 -0.9

Or see this region's nucleotide sequence