Experiment: copper (II) chloride 0.32 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspG and gspH are separated by 1 nucleotides gspH and gspI overlap by 14 nucleotides gspI and gspJ overlap by 20 nucleotides gspJ and gspK overlap by 4 nucleotides
SO0169: gspG - general secretion pathway protein G (NCBI ptt file), at 177,279 to 177,713
gspG
SO0170: gspH - general secretion pathway protein H (NCBI ptt file), at 177,715 to 178,287
gspH
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385
gspK
Position (kb) 178
179 Strain fitness (log2 ratio) -2
-1
0
1 at 177.277 kb on - strand at 177.303 kb on + strand at 177.303 kb on + strand at 177.329 kb on + strand, within gspG at 177.364 kb on - strand, within gspG at 177.366 kb on - strand, within gspG at 177.397 kb on - strand, within gspG at 177.476 kb on + strand, within gspG at 177.492 kb on + strand, within gspG at 177.554 kb on + strand, within gspG at 177.613 kb on - strand, within gspG at 177.638 kb on + strand, within gspG at 177.662 kb on + strand, within gspG at 177.751 kb on - strand at 177.788 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.845 kb on - strand, within gspH at 177.845 kb on - strand, within gspH at 178.065 kb on - strand, within gspH at 178.221 kb on - strand, within gspH at 178.237 kb on + strand at 178.266 kb on - strand at 178.282 kb on + strand at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ at 178.730 kb on - strand, within gspJ at 178.742 kb on - strand, within gspJ at 178.744 kb on + strand, within gspJ at 178.816 kb on + strand, within gspJ at 178.824 kb on - strand, within gspJ at 178.849 kb on - strand, within gspJ at 178.850 kb on + strand, within gspJ at 178.867 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.950 kb on - strand, within gspJ at 178.951 kb on - strand, within gspJ at 179.018 kb on - strand, within gspJ at 179.151 kb on + strand, within gspJ at 179.157 kb on + strand, within gspJ at 179.220 kb on - strand, within gspJ at 179.273 kb on + strand, within gspJ at 179.303 kb on - strand, within gspJ at 179.394 kb on + strand at 179.436 kb on - strand at 179.544 kb on - strand, within gspK at 179.635 kb on + strand, within gspK
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 0.32 mM remove 177,277 - -2.6 177,303 + -1.0 177,303 + -0.8 177,329 + gspG SO0169 0.11 -1.4 177,364 - gspG SO0169 0.20 -1.7 177,366 - gspG SO0169 0.20 -0.6 177,397 - gspG SO0169 0.27 -1.6 177,476 + gspG SO0169 0.45 -1.4 177,492 + gspG SO0169 0.49 -1.7 177,554 + gspG SO0169 0.63 -1.0 177,613 - gspG SO0169 0.77 +0.2 177,638 + gspG SO0169 0.83 -1.4 177,662 + gspG SO0169 0.88 -0.4 177,751 - -0.7 177,788 + gspH SO0170 0.13 -1.5 177,837 + gspH SO0170 0.21 -2.3 177,837 + gspH SO0170 0.21 +0.7 177,837 + gspH SO0170 0.21 -0.5 177,845 - gspH SO0170 0.23 -0.8 177,845 - gspH SO0170 0.23 -1.5 178,065 - gspH SO0170 0.61 -2.4 178,221 - gspH SO0170 0.88 -0.6 178,237 + -0.4 178,266 - -0.7 178,282 + -0.3 178,440 + gspI SO0171 0.45 -1.4 178,516 - gspI SO0171 0.66 -0.0 178,605 - gspI SO0171 0.90 -2.2 178,654 + +0.1 178,654 + +0.1 178,654 + -1.1 178,654 + -0.6 178,654 + -0.9 178,654 + -1.1 178,662 - -0.6 178,662 - -1.5 178,700 + gspJ SO0172 0.10 -0.2 178,730 - gspJ SO0172 0.14 -0.6 178,742 - gspJ SO0172 0.15 -0.6 178,744 + gspJ SO0172 0.16 -1.4 178,816 + gspJ SO0172 0.25 -0.1 178,824 - gspJ SO0172 0.26 -1.6 178,849 - gspJ SO0172 0.29 -1.0 178,850 + gspJ SO0172 0.30 -0.5 178,867 + gspJ SO0172 0.32 -0.9 178,943 + gspJ SO0172 0.42 -1.4 178,943 + gspJ SO0172 0.42 -2.0 178,950 - gspJ SO0172 0.43 -1.1 178,951 - gspJ SO0172 0.43 -0.7 179,018 - gspJ SO0172 0.51 -1.8 179,151 + gspJ SO0172 0.69 -0.8 179,157 + gspJ SO0172 0.70 -1.1 179,220 - gspJ SO0172 0.78 -0.8 179,273 + gspJ SO0172 0.85 -0.8 179,303 - gspJ SO0172 0.89 -1.1 179,394 + -0.4 179,436 - -0.7 179,544 - gspK SO0173 0.16 -1.1 179,635 + gspK SO0173 0.25 -0.3
Or see this region's nucleotide sequence