Experiment: MoYLS4 with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1999 and DvMF_2000 are separated by 465 nucleotides DvMF_2000 and DvMF_2001 overlap by 8 nucleotides DvMF_2001 and DvMF_2002 are separated by 63 nucleotides
DvMF_1999: DvMF_1999 - protein of unknown function DUF112 transmembrane (RefSeq), at 2,477,896 to 2,479,440
_1999
DvMF_2000: DvMF_2000 - hypothetical protein (RefSeq), at 2,479,906 to 2,480,367
_2000
DvMF_2001: DvMF_2001 - protein of unknown function DUF112 transmembrane (RefSeq), at 2,480,360 to 2,481,904
_2001
DvMF_2002: DvMF_2002 - hypothetical protein (RefSeq), at 2,481,968 to 2,482,930
_2002
Position (kb)
2480
2481
2482 Strain fitness (log2 ratio)
-1
0
1 at 2479.458 kb on - strand at 2479.478 kb on - strand at 2479.581 kb on + strand at 2479.647 kb on + strand at 2479.918 kb on + strand at 2479.975 kb on + strand, within DvMF_2000 at 2480.021 kb on + strand, within DvMF_2000 at 2480.043 kb on - strand, within DvMF_2000 at 2480.131 kb on + strand, within DvMF_2000 at 2480.139 kb on - strand, within DvMF_2000 at 2480.341 kb on + strand at 2480.379 kb on + strand at 2480.379 kb on + strand at 2480.482 kb on - strand at 2480.738 kb on + strand, within DvMF_2001 at 2480.738 kb on + strand, within DvMF_2001 at 2480.874 kb on - strand, within DvMF_2001 at 2480.874 kb on - strand, within DvMF_2001 at 2481.220 kb on - strand, within DvMF_2001 at 2481.256 kb on + strand, within DvMF_2001 at 2481.516 kb on + strand, within DvMF_2001 at 2481.576 kb on - strand, within DvMF_2001 at 2481.678 kb on + strand, within DvMF_2001 at 2481.914 kb on + strand at 2482.161 kb on + strand, within DvMF_2002 at 2482.198 kb on + strand, within DvMF_2002 at 2482.209 kb on - strand, within DvMF_2002 at 2482.371 kb on + strand, within DvMF_2002 at 2482.382 kb on - strand, within DvMF_2002 at 2482.440 kb on + strand, within DvMF_2002 at 2482.462 kb on - strand, within DvMF_2002 at 2482.472 kb on - strand, within DvMF_2002 at 2482.584 kb on - strand, within DvMF_2002 at 2482.666 kb on - strand, within DvMF_2002 at 2482.815 kb on + strand, within DvMF_2002 at 2482.870 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/ml remove 2,479,458 - +0.0 2,479,478 - +0.2 2,479,581 + -0.6 2,479,647 + +0.5 2,479,918 + -0.0 2,479,975 + DvMF_2000 0.15 -0.1 2,480,021 + DvMF_2000 0.25 -0.6 2,480,043 - DvMF_2000 0.30 -0.4 2,480,131 + DvMF_2000 0.49 -0.8 2,480,139 - DvMF_2000 0.50 -0.1 2,480,341 + +0.0 2,480,379 + -0.3 2,480,379 + -0.2 2,480,482 - -0.0 2,480,738 + DvMF_2001 0.24 +0.2 2,480,738 + DvMF_2001 0.24 +0.2 2,480,874 - DvMF_2001 0.33 -0.0 2,480,874 - DvMF_2001 0.33 -0.1 2,481,220 - DvMF_2001 0.56 -0.1 2,481,256 + DvMF_2001 0.58 -0.1 2,481,516 + DvMF_2001 0.75 +0.0 2,481,576 - DvMF_2001 0.79 -0.1 2,481,678 + DvMF_2001 0.85 -0.1 2,481,914 + -0.3 2,482,161 + DvMF_2002 0.20 -0.3 2,482,198 + DvMF_2002 0.24 +0.1 2,482,209 - DvMF_2002 0.25 -0.2 2,482,371 + DvMF_2002 0.42 -0.1 2,482,382 - DvMF_2002 0.43 -0.1 2,482,440 + DvMF_2002 0.49 -0.0 2,482,462 - DvMF_2002 0.51 -0.4 2,482,472 - DvMF_2002 0.52 -0.0 2,482,584 - DvMF_2002 0.64 +0.7 2,482,666 - DvMF_2002 0.72 -0.0 2,482,815 + DvMF_2002 0.88 -0.1 2,482,870 + +0.0
Or see this region's nucleotide sequence