Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2199 and Shew_2200 are separated by 64 nucleotides Shew_2200 and hisH overlap by 4 nucleotides hisH and Shew_2202 overlap by 4 nucleotides
Shew_2199: Shew_2199 - histidinol-phosphate aminotransferase (RefSeq), at 2,559,814 to 2,560,896
_2199
Shew_2200: Shew_2200 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,560,961 to 2,562,031
_2200
Shew_2201: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,562,028 to 2,562,702
hisH
Shew_2202: Shew_2202 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,562,699 to 2,563,436
_2202
Position (kb)
2560
2561
2562
2563 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 2560.312 kb on + strand, within Shew_2199 at 2560.511 kb on + strand, within Shew_2199 at 2560.519 kb on - strand, within Shew_2199 at 2560.638 kb on + strand, within Shew_2199 at 2560.881 kb on + strand at 2561.293 kb on + strand, within Shew_2200 at 2561.399 kb on + strand, within Shew_2200 at 2561.417 kb on + strand, within Shew_2200 at 2561.425 kb on - strand, within Shew_2200 at 2561.499 kb on + strand, within Shew_2200 at 2561.750 kb on + strand, within Shew_2200 at 2561.758 kb on - strand, within Shew_2200 at 2562.347 kb on + strand, within hisH at 2562.722 kb on + strand at 2562.800 kb on + strand, within Shew_2202
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 2,560,312 + Shew_2199 0.46 -2.4 2,560,511 + Shew_2199 0.64 -4.1 2,560,519 - Shew_2199 0.65 -4.8 2,560,638 + Shew_2199 0.76 -3.6 2,560,881 + -2.2 2,561,293 + Shew_2200 0.31 -4.7 2,561,399 + Shew_2200 0.41 -5.3 2,561,417 + Shew_2200 0.43 -4.0 2,561,425 - Shew_2200 0.43 -5.3 2,561,499 + Shew_2200 0.50 -7.8 2,561,750 + Shew_2200 0.74 -7.5 2,561,758 - Shew_2200 0.74 -3.7 2,562,347 + hisH Shew_2201 0.47 -7.4 2,562,722 + -7.3 2,562,800 + Shew_2202 0.14 -3.6
Or see this region's nucleotide sequence