Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1998 and Shew_1999 are separated by 62 nucleotides Shew_1999 and Shew_R0065 are separated by 395 nucleotides Shew_R0065 and Shew_R0066 are separated by 38 nucleotides Shew_R0066 and Shew_R0067 are separated by 35 nucleotides Shew_R0067 and Shew_2000 are separated by 233 nucleotides
Shew_1998: Shew_1998 - protein of unknown function UPF0052 and CofD (RefSeq), at 2,309,364 to 2,310,275
_1998
Shew_1999: Shew_1999 - aromatic amino acid aminotransferase (RefSeq), at 2,310,338 to 2,311,531
_1999
Shew_R0065: Shew_R0065 - tRNA-Leu (RefSeq), at 2,311,927 to 2,312,013
_R0065
Shew_R0066: Shew_R0066 - tRNA-Cys (RefSeq), at 2,312,052 to 2,312,125
_R0066
Shew_R0067: Shew_R0067 - tRNA-Gly (RefSeq), at 2,312,161 to 2,312,236
_R0067
Shew_2000: Shew_2000 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,312,470 to 2,313,906
_2000
Position (kb)
2310
2311
2312 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2309.834 kb on - strand, within Shew_1998 at 2310.407 kb on - strand at 2310.849 kb on + strand, within Shew_1999 at 2311.513 kb on - strand at 2311.574 kb on + strand at 2311.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 2,309,834 - Shew_1998 0.52 +0.6 2,310,407 - -3.0 2,310,849 + Shew_1999 0.43 -2.7 2,311,513 - -1.3 2,311,574 + -0.3 2,311,574 + -0.1
Or see this region's nucleotide sequence