Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1811 and Shew_R0057 are separated by 814 nucleotides Shew_R0057 and Shew_R0058 are separated by 46 nucleotides Shew_R0058 and Shew_R0059 are separated by 47 nucleotides Shew_R0059 and Shew_R0060 are separated by 47 nucleotides Shew_R0060 and Shew_1812 are separated by 270 nucleotides
Shew_1811: Shew_1811 - hypothetical protein (RefSeq), at 2,080,969 to 2,081,502
_1811
Shew_R0057: Shew_R0057 - tRNA-Met (RefSeq), at 2,082,317 to 2,082,393
_R0057
Shew_R0058: Shew_R0058 - tRNA-Met (RefSeq), at 2,082,440 to 2,082,516
_R0058
Shew_R0059: Shew_R0059 - tRNA-Met (RefSeq), at 2,082,564 to 2,082,640
_R0059
Shew_R0060: Shew_R0060 - tRNA-Met (RefSeq), at 2,082,688 to 2,082,764
_R0060
Shew_1812: Shew_1812 - 23S rRNA m(2)G2445 methyltransferase (RefSeq), at 2,083,035 to 2,085,170
_1812
Position (kb)
2082
2083 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2081.438 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.499 kb on - strand at 2081.770 kb on - strand at 2081.813 kb on + strand at 2081.813 kb on + strand at 2081.821 kb on + strand at 2081.821 kb on + strand at 2081.821 kb on - strand at 2081.821 kb on + strand at 2081.939 kb on - strand at 2083.137 kb on + strand at 2083.154 kb on + strand at 2083.169 kb on + strand at 2083.171 kb on + strand at 2083.247 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 2,081,438 + Shew_1811 0.88 -0.6 2,081,438 + Shew_1811 0.88 +0.4 2,081,438 + Shew_1811 0.88 -0.5 2,081,499 - +1.8 2,081,770 - -1.3 2,081,813 + +0.2 2,081,813 + -0.6 2,081,821 + -0.2 2,081,821 + +2.9 2,081,821 - -0.0 2,081,821 + +2.2 2,081,939 - -0.5 2,083,137 + +0.3 2,083,154 + +0.6 2,083,169 + +0.0 2,083,171 + +0.3 2,083,247 + +0.0
Or see this region's nucleotide sequence