Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1209 and Shew_1210 overlap by 8 nucleotides Shew_1210 and Shew_1211 overlap by 19 nucleotides Shew_1211 and Shew_1212 are separated by 49 nucleotides
Shew_1209: Shew_1209 - pseudouridylate synthase (RefSeq), at 1,403,308 to 1,404,375
_1209
Shew_1210: Shew_1210 - stationary-phase survival protein SurE (RefSeq), at 1,404,368 to 1,405,117
_1210
Shew_1211: Shew_1211 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,405,099 to 1,405,749
_1211
Shew_1212: Shew_1212 - peptidase M23B (RefSeq), at 1,405,799 to 1,406,731
_1212
Position (kb) 1405
1406 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1404.427 kb on + strand at 1404.481 kb on + strand, within Shew_1210 at 1404.569 kb on - strand, within Shew_1210 at 1405.267 kb on + strand, within Shew_1211 at 1405.302 kb on + strand, within Shew_1211 at 1405.310 kb on - strand, within Shew_1211 at 1405.544 kb on + strand, within Shew_1211 at 1405.995 kb on + strand, within Shew_1212 at 1406.079 kb on - strand, within Shew_1212 at 1406.562 kb on + strand, within Shew_1212
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 1,404,427 + -1.0 1,404,481 + Shew_1210 0.15 -0.3 1,404,569 - Shew_1210 0.27 +0.3 1,405,267 + Shew_1211 0.26 -1.4 1,405,302 + Shew_1211 0.31 -0.5 1,405,310 - Shew_1211 0.32 -0.4 1,405,544 + Shew_1211 0.68 -1.0 1,405,995 + Shew_1212 0.21 -0.3 1,406,079 - Shew_1212 0.30 -3.8 1,406,562 + Shew_1212 0.82 -0.1
Or see this region's nucleotide sequence