Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0216 and Shew_0217 are separated by 74 nucleotides Shew_0217 and Shew_0218 are separated by 8 nucleotides Shew_0218 and Shew_0219 are separated by 211 nucleotides Shew_0219 and Shew_0220 are separated by 2 nucleotides Shew_0220 and Shew_0221 are separated by 5 nucleotides
Shew_0216: Shew_0216 - DNA adenine methylase (RefSeq), at 231,996 to 232,835
_0216
Shew_0217: Shew_0217 - hypothetical protein (RefSeq), at 232,910 to 233,083
_0217
Shew_0218: Shew_0218 - hypothetical protein (RefSeq), at 233,092 to 233,436
_0218
Shew_0219: Shew_0219 - ribulose-phosphate 3-epimerase (RefSeq), at 233,648 to 234,322
_0219
Shew_0220: Shew_0220 - phosphoglycolate phosphatase (RefSeq), at 234,325 to 235,020
_0220
Shew_0221: Shew_0221 - tryptophanyl-tRNA synthetase (RefSeq), at 235,026 to 236,024
_0221
Position (kb)
233
234
235 Strain fitness (log2 ratio)
-1
0
1
2
3 at 232.877 kb on + strand at 232.981 kb on + strand, within Shew_0217 at 233.086 kb on - strand at 233.147 kb on + strand, within Shew_0218 at 233.194 kb on + strand, within Shew_0218 at 233.370 kb on - strand, within Shew_0218 at 233.425 kb on - strand at 234.312 kb on - strand at 234.312 kb on - strand at 234.411 kb on + strand, within Shew_0220 at 234.545 kb on - strand, within Shew_0220 at 234.744 kb on + strand, within Shew_0220 at 234.798 kb on + strand, within Shew_0220 at 234.806 kb on - strand, within Shew_0220
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 232,877 + -1.3 232,981 + Shew_0217 0.41 -0.8 233,086 - -0.5 233,147 + Shew_0218 0.16 -1.1 233,194 + Shew_0218 0.30 -1.0 233,370 - Shew_0218 0.81 +0.3 233,425 - +0.4 234,312 - +1.3 234,312 - +1.4 234,411 + Shew_0220 0.12 +2.4 234,545 - Shew_0220 0.32 +3.2 234,744 + Shew_0220 0.60 +2.1 234,798 + Shew_0220 0.68 +2.7 234,806 - Shew_0220 0.69 +2.4
Or see this region's nucleotide sequence