Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_91 and HP15_92 are separated by 22 nucleotides HP15_92 and HP15_93 are separated by 26 nucleotides HP15_93 and HP15_94 are separated by 42 nucleotides HP15_94 and HP15_95 are separated by 75 nucleotides
HP15_91: HP15_91 - conserved hypothetical protein, membrane, at 95,809 to 96,651
_91
HP15_92: HP15_92 - DSBA oxidoreductase, at 96,674 to 97,312
_92
HP15_93: HP15_93 - hypothetical protein, at 97,339 to 97,446
_93
HP15_94: HP15_94 - sodium: bile acid symporter family protein-like protein, at 97,489 to 98,061
_94
HP15_95: HP15_95 - cation diffusion facilitator family transporter, at 98,137 to 98,925
_95
Position (kb)
97
98 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 96.371 kb on + strand, within HP15_91 at 96.371 kb on + strand, within HP15_91 at 96.379 kb on - strand, within HP15_91 at 96.398 kb on - strand, within HP15_91 at 96.450 kb on - strand, within HP15_91 at 96.469 kb on + strand, within HP15_91 at 96.480 kb on + strand, within HP15_91 at 96.480 kb on + strand, within HP15_91 at 96.488 kb on - strand, within HP15_91 at 96.498 kb on + strand, within HP15_91 at 96.506 kb on - strand, within HP15_91 at 96.506 kb on - strand, within HP15_91 at 96.527 kb on + strand, within HP15_91 at 96.531 kb on - strand, within HP15_91 at 96.538 kb on + strand, within HP15_91 at 96.541 kb on - strand, within HP15_91 at 96.682 kb on - strand at 96.691 kb on + strand at 96.693 kb on - strand at 96.707 kb on + strand at 96.723 kb on - strand at 96.723 kb on - strand at 96.754 kb on + strand, within HP15_92 at 96.794 kb on - strand, within HP15_92 at 96.835 kb on + strand, within HP15_92 at 96.840 kb on + strand, within HP15_92 at 96.844 kb on - strand, within HP15_92 at 96.845 kb on + strand, within HP15_92 at 96.904 kb on + strand, within HP15_92 at 96.912 kb on - strand, within HP15_92 at 96.913 kb on + strand, within HP15_92 at 96.928 kb on - strand, within HP15_92 at 96.947 kb on + strand, within HP15_92 at 96.955 kb on - strand, within HP15_92 at 97.014 kb on - strand, within HP15_92 at 97.032 kb on - strand, within HP15_92 at 97.034 kb on + strand, within HP15_92 at 97.039 kb on + strand, within HP15_92 at 97.053 kb on - strand, within HP15_92 at 97.088 kb on + strand, within HP15_92 at 97.158 kb on + strand, within HP15_92 at 97.305 kb on - strand at 97.305 kb on - strand at 97.310 kb on + strand at 97.336 kb on + strand at 97.429 kb on + strand, within HP15_93 at 97.457 kb on + strand at 97.457 kb on + strand at 97.465 kb on - strand at 97.494 kb on - strand at 97.576 kb on + strand, within HP15_94 at 97.649 kb on - strand, within HP15_94 at 97.649 kb on - strand, within HP15_94 at 97.697 kb on - strand, within HP15_94 at 97.746 kb on - strand, within HP15_94 at 97.796 kb on - strand, within HP15_94 at 97.796 kb on - strand, within HP15_94 at 97.915 kb on + strand, within HP15_94 at 97.949 kb on + strand, within HP15_94 at 97.957 kb on + strand, within HP15_94 at 97.957 kb on + strand, within HP15_94 at 97.957 kb on + strand, within HP15_94 at 97.959 kb on + strand, within HP15_94 at 97.965 kb on - strand, within HP15_94 at 97.965 kb on - strand, within HP15_94 at 97.967 kb on - strand, within HP15_94 at 97.967 kb on - strand, within HP15_94 at 97.967 kb on - strand, within HP15_94 at 97.985 kb on - strand, within HP15_94 at 98.027 kb on + strand at 98.027 kb on + strand at 98.027 kb on + strand at 98.027 kb on + strand at 98.035 kb on - strand at 98.065 kb on - strand at 98.067 kb on + strand at 98.067 kb on + strand at 98.075 kb on - strand at 98.134 kb on - strand at 98.145 kb on + strand at 98.245 kb on + strand, within HP15_95 at 98.304 kb on - strand, within HP15_95 at 98.342 kb on + strand, within HP15_95 at 98.411 kb on + strand, within HP15_95 at 98.411 kb on + strand, within HP15_95 at 98.414 kb on - strand, within HP15_95 at 98.427 kb on + strand, within HP15_95 at 98.429 kb on - strand, within HP15_95
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 96,371 + HP15_91 0.67 +0.2 96,371 + HP15_91 0.67 +1.5 96,379 - HP15_91 0.68 +1.5 96,398 - HP15_91 0.70 +0.3 96,450 - HP15_91 0.76 -0.7 96,469 + HP15_91 0.78 -0.3 96,480 + HP15_91 0.80 -0.5 96,480 + HP15_91 0.80 -1.0 96,488 - HP15_91 0.81 -0.2 96,498 + HP15_91 0.82 +0.8 96,506 - HP15_91 0.83 -0.3 96,506 - HP15_91 0.83 -0.6 96,527 + HP15_91 0.85 -2.0 96,531 - HP15_91 0.86 +0.7 96,538 + HP15_91 0.86 +0.3 96,541 - HP15_91 0.87 -2.2 96,682 - +1.6 96,691 + -1.5 96,693 - -0.5 96,707 + -0.4 96,723 - +0.4 96,723 - -0.5 96,754 + HP15_92 0.13 +1.8 96,794 - HP15_92 0.19 +1.6 96,835 + HP15_92 0.25 -1.1 96,840 + HP15_92 0.26 -0.1 96,844 - HP15_92 0.27 +0.6 96,845 + HP15_92 0.27 +1.7 96,904 + HP15_92 0.36 -0.2 96,912 - HP15_92 0.37 +1.9 96,913 + HP15_92 0.37 +0.4 96,928 - HP15_92 0.40 +0.8 96,947 + HP15_92 0.43 +0.6 96,955 - HP15_92 0.44 -0.5 97,014 - HP15_92 0.53 -0.2 97,032 - HP15_92 0.56 -1.6 97,034 + HP15_92 0.56 +0.8 97,039 + HP15_92 0.57 -0.1 97,053 - HP15_92 0.59 -0.8 97,088 + HP15_92 0.65 +0.6 97,158 + HP15_92 0.76 +1.8 97,305 - +0.8 97,305 - -1.0 97,310 + +1.3 97,336 + -1.6 97,429 + HP15_93 0.83 +1.1 97,457 + -0.2 97,457 + -1.1 97,465 - -0.0 97,494 - -0.3 97,576 + HP15_94 0.15 +0.4 97,649 - HP15_94 0.28 +1.0 97,649 - HP15_94 0.28 +0.3 97,697 - HP15_94 0.36 +0.1 97,746 - HP15_94 0.45 -1.0 97,796 - HP15_94 0.54 +0.1 97,796 - HP15_94 0.54 +1.5 97,915 + HP15_94 0.74 -0.9 97,949 + HP15_94 0.80 +1.8 97,957 + HP15_94 0.82 -0.5 97,957 + HP15_94 0.82 -0.6 97,957 + HP15_94 0.82 +0.3 97,959 + HP15_94 0.82 -0.4 97,965 - HP15_94 0.83 +0.4 97,965 - HP15_94 0.83 +0.0 97,967 - HP15_94 0.83 +1.8 97,967 - HP15_94 0.83 +1.5 97,967 - HP15_94 0.83 -0.0 97,985 - HP15_94 0.87 +0.3 98,027 + +2.1 98,027 + +1.9 98,027 + +0.1 98,027 + -0.1 98,035 - +0.5 98,065 - -0.6 98,067 + +1.0 98,067 + +1.5 98,075 - +1.1 98,134 - +1.8 98,145 + +2.1 98,245 + HP15_95 0.14 -0.7 98,304 - HP15_95 0.21 -0.4 98,342 + HP15_95 0.26 +0.1 98,411 + HP15_95 0.35 -0.3 98,411 + HP15_95 0.35 +1.3 98,414 - HP15_95 0.35 -0.5 98,427 + HP15_95 0.37 +1.9 98,429 - HP15_95 0.37 -0.1
Or see this region's nucleotide sequence