Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1004 and HP15_1005 are separated by 207 nucleotides HP15_1005 and HP15_1006 are separated by 29 nucleotides HP15_1006 and HP15_1007 are separated by 104 nucleotides
HP15_1004: HP15_1004 - 3-methylcrotonyl CoA carboxylase, beta subunit, at 1,014,240 to 1,015,847
_1004
HP15_1005: HP15_1005 - acyl-CoA dehydrogenase domain protein, at 1,016,055 to 1,017,224
_1005
HP15_1006: HP15_1006 - transcriptional regulator, MerR family, at 1,017,254 to 1,017,697
_1006
HP15_1007: HP15_1007 - 3-hydroxyacyl-CoA dehydrogenase type II, at 1,017,802 to 1,018,563
_1007
Position (kb) 1016
1017
1018 Strain fitness (log2 ratio) -1
0
1 at 1016.364 kb on - strand, within HP15_1005 at 1017.597 kb on + strand, within HP15_1006 at 1017.605 kb on - strand, within HP15_1006 at 1017.616 kb on - strand, within HP15_1006 at 1017.847 kb on + strand at 1017.856 kb on - strand at 1017.919 kb on + strand, within HP15_1007 at 1018.002 kb on + strand, within HP15_1007 at 1018.052 kb on + strand, within HP15_1007 at 1018.177 kb on + strand, within HP15_1007
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 1,016,364 - HP15_1005 0.26 +1.7 1,017,597 + HP15_1006 0.77 -1.0 1,017,605 - HP15_1006 0.79 -0.6 1,017,616 - HP15_1006 0.82 +0.2 1,017,847 + +1.6 1,017,856 - -0.5 1,017,919 + HP15_1007 0.15 +0.5 1,018,002 + HP15_1007 0.26 -0.8 1,018,052 + HP15_1007 0.33 +1.5 1,018,177 + HP15_1007 0.49 -0.7
Or see this region's nucleotide sequence