Experiment: D-Maltose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2639 and purU are separated by 108 nucleotides purU and Shew_2641 are separated by 33 nucleotides Shew_2641 and Shew_2642 overlap by 49 nucleotides Shew_2642 and Shew_2643 are separated by 373 nucleotides
Shew_2639: Shew_2639 - hypothetical protein (RefSeq), at 3,115,199 to 3,115,543
_2639
Shew_2640: purU - formyltetrahydrofolate deformylase (RefSeq), at 3,115,652 to 3,116,485
purU
Shew_2641: Shew_2641 - PTS system, glucose-like IIB subunint (RefSeq), at 3,116,519 to 3,117,991
_2641
Shew_2642: Shew_2642 - flavodoxin (RefSeq), at 3,117,943 to 3,118,407
_2642
Shew_2643: Shew_2643 - pseudouridylate synthase (RefSeq), at 3,118,781 to 3,119,602
_2643
Position (kb)
3116
3117
3118 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3115.652 kb on - strand at 3115.713 kb on + strand at 3115.721 kb on - strand at 3115.882 kb on + strand, within purU at 3116.071 kb on - strand, within purU at 3116.127 kb on - strand, within purU at 3116.421 kb on + strand at 3116.421 kb on + strand at 3116.429 kb on - strand at 3116.440 kb on + strand at 3116.448 kb on - strand at 3116.945 kb on + strand, within Shew_2641 at 3117.285 kb on - strand, within Shew_2641 at 3117.459 kb on + strand, within Shew_2641 at 3118.241 kb on - strand, within Shew_2642
Per-strain Table
Position Strand Gene LocusTag Fraction D-Maltose (C) remove 3,115,652 - -0.3 3,115,713 + +0.5 3,115,721 - -3.1 3,115,882 + purU Shew_2640 0.28 -1.3 3,116,071 - purU Shew_2640 0.50 -2.2 3,116,127 - purU Shew_2640 0.57 -2.6 3,116,421 + -1.2 3,116,421 + -2.6 3,116,429 - -2.4 3,116,440 + -2.0 3,116,448 - -2.3 3,116,945 + Shew_2641 0.29 -0.7 3,117,285 - Shew_2641 0.52 -1.3 3,117,459 + Shew_2641 0.64 +0.2 3,118,241 - Shew_2642 0.64 -0.1
Or see this region's nucleotide sequence