Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_76 and HP15_77 are separated by 443 nucleotides HP15_77 and HP15_78 are separated by 68 nucleotides HP15_78 and HP15_79 are separated by 85 nucleotides
HP15_76: HP15_76 - phage integrase family protein-like protein, at 84,123 to 87,473
_76
HP15_77: HP15_77 - hypothetical protein, at 87,917 to 88,117
_77
HP15_78: HP15_78 - hypothetical protein, at 88,186 to 88,380
_78
HP15_79: HP15_79 - phage integrase family protein, at 88,466 to 89,674
_79
Position (kb)
88
89 Strain fitness (log2 ratio)
-1
0
1 at 87.351 kb on + strand at 87.359 kb on + strand at 87.448 kb on - strand at 87.500 kb on - strand at 87.537 kb on - strand at 87.617 kb on + strand at 87.617 kb on + strand at 87.717 kb on - strand at 87.854 kb on - strand at 87.872 kb on + strand at 88.120 kb on + strand at 88.120 kb on + strand at 88.120 kb on + strand at 88.128 kb on - strand at 88.149 kb on + strand at 88.152 kb on - strand at 88.197 kb on + strand at 88.252 kb on - strand, within HP15_78 at 88.292 kb on - strand, within HP15_78 at 88.292 kb on - strand, within HP15_78 at 88.380 kb on + strand at 88.423 kb on - strand at 88.463 kb on + strand at 88.610 kb on + strand, within HP15_79 at 88.613 kb on - strand, within HP15_79 at 88.636 kb on + strand, within HP15_79 at 88.732 kb on - strand, within HP15_79 at 88.732 kb on - strand, within HP15_79 at 88.756 kb on - strand, within HP15_79 at 88.814 kb on + strand, within HP15_79 at 88.986 kb on + strand, within HP15_79 at 89.021 kb on - strand, within HP15_79 at 89.063 kb on - strand, within HP15_79 at 89.100 kb on + strand, within HP15_79 at 89.110 kb on + strand, within HP15_79
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 87,351 + -0.2 87,359 + +0.1 87,448 - +1.2 87,500 - +0.8 87,537 - -0.1 87,617 + -0.1 87,617 + -0.2 87,717 - +0.2 87,854 - +0.6 87,872 + +0.3 88,120 + +0.1 88,120 + -0.1 88,120 + +0.6 88,128 - -0.2 88,149 + +0.0 88,152 - +1.0 88,197 + +0.0 88,252 - HP15_78 0.34 -0.3 88,292 - HP15_78 0.54 -0.7 88,292 - HP15_78 0.54 -1.4 88,380 + -0.0 88,423 - -0.3 88,463 + -0.8 88,610 + HP15_79 0.12 -0.7 88,613 - HP15_79 0.12 +0.5 88,636 + HP15_79 0.14 +0.0 88,732 - HP15_79 0.22 -1.0 88,732 - HP15_79 0.22 -0.3 88,756 - HP15_79 0.24 -0.2 88,814 + HP15_79 0.29 -0.1 88,986 + HP15_79 0.43 +0.1 89,021 - HP15_79 0.46 -0.0 89,063 - HP15_79 0.49 +0.8 89,100 + HP15_79 0.52 -0.5 89,110 + HP15_79 0.53 +1.1
Or see this region's nucleotide sequence