Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_654 and HP15_655 are separated by 90 nucleotides HP15_655 and HP15_656 are separated by 28 nucleotides HP15_656 and HP15_657 are separated by 9 nucleotides HP15_657 and HP15_658 overlap by 4 nucleotides
HP15_654: HP15_654 - major tail sheath protein, at 675,391 to 676,908
_654
HP15_655: HP15_655 - phage tail collar domain protein-like protein, at 676,999 to 677,463
_655
HP15_656: HP15_656 - tail fiber assembly protein-like protein, at 677,492 to 677,773
_656
HP15_657: HP15_657 - phage tail fiber protein, at 677,783 to 679,219
_657
HP15_658: HP15_658 - secreted protein-putative xanthan lyase, at 679,216 to 681,789
_658
Position (kb) 677
678
679
680 Strain fitness (log2 ratio) -1
0
1
2 at 676.881 kb on + strand at 676.881 kb on + strand at 676.889 kb on - strand at 676.889 kb on - strand at 676.935 kb on - strand at 677.150 kb on - strand, within HP15_655 at 677.249 kb on - strand, within HP15_655 at 677.249 kb on - strand, within HP15_655 at 677.326 kb on - strand, within HP15_655 at 677.405 kb on - strand, within HP15_655 at 677.434 kb on - strand at 677.439 kb on - strand at 677.498 kb on - strand at 677.545 kb on - strand, within HP15_656 at 677.582 kb on - strand, within HP15_656 at 677.582 kb on - strand, within HP15_656 at 677.733 kb on + strand, within HP15_656 at 677.736 kb on - strand, within HP15_656 at 677.793 kb on + strand at 677.793 kb on + strand at 677.865 kb on - strand at 678.000 kb on + strand, within HP15_657 at 678.040 kb on - strand, within HP15_657 at 678.052 kb on - strand, within HP15_657 at 678.092 kb on + strand, within HP15_657 at 678.130 kb on - strand, within HP15_657 at 678.130 kb on - strand, within HP15_657 at 678.146 kb on - strand, within HP15_657 at 678.201 kb on + strand, within HP15_657 at 678.273 kb on - strand, within HP15_657 at 678.319 kb on - strand, within HP15_657 at 678.333 kb on - strand, within HP15_657 at 678.409 kb on + strand, within HP15_657 at 678.409 kb on - strand, within HP15_657 at 678.466 kb on + strand, within HP15_657 at 678.479 kb on - strand, within HP15_657 at 678.500 kb on + strand, within HP15_657 at 678.505 kb on + strand, within HP15_657 at 678.515 kb on + strand, within HP15_657 at 678.560 kb on - strand, within HP15_657 at 678.624 kb on + strand, within HP15_657 at 678.624 kb on + strand, within HP15_657 at 678.664 kb on + strand, within HP15_657 at 678.664 kb on + strand, within HP15_657 at 678.779 kb on - strand, within HP15_657 at 678.826 kb on + strand, within HP15_657 at 678.853 kb on + strand, within HP15_657 at 678.900 kb on - strand, within HP15_657 at 678.964 kb on + strand, within HP15_657 at 678.995 kb on - strand, within HP15_657 at 679.085 kb on + strand at 679.107 kb on - strand at 679.233 kb on + strand at 679.244 kb on + strand at 679.312 kb on + strand at 679.463 kb on + strand at 679.619 kb on - strand, within HP15_658 at 679.798 kb on - strand, within HP15_658 at 679.825 kb on + strand, within HP15_658 at 679.833 kb on - strand, within HP15_658 at 679.833 kb on - strand, within HP15_658 at 679.833 kb on - strand, within HP15_658 at 679.925 kb on + strand, within HP15_658 at 679.942 kb on + strand, within HP15_658 at 679.942 kb on + strand, within HP15_658 at 679.950 kb on - strand, within HP15_658 at 679.950 kb on - strand, within HP15_658 at 679.965 kb on - strand, within HP15_658 at 680.160 kb on - strand, within HP15_658 at 680.160 kb on - strand, within HP15_658 at 680.166 kb on - strand, within HP15_658 at 680.199 kb on + strand, within HP15_658 at 680.199 kb on + strand, within HP15_658 at 680.199 kb on + strand, within HP15_658 at 680.207 kb on - strand, within HP15_658
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 676,881 + -0.2 676,881 + -0.5 676,889 - +0.4 676,889 - -0.2 676,935 - -0.1 677,150 - HP15_655 0.32 -0.3 677,249 - HP15_655 0.54 +0.5 677,249 - HP15_655 0.54 +1.5 677,326 - HP15_655 0.70 +0.7 677,405 - HP15_655 0.87 +0.3 677,434 - +1.4 677,439 - +1.2 677,498 - +0.2 677,545 - HP15_656 0.19 +0.4 677,582 - HP15_656 0.32 +0.1 677,582 - HP15_656 0.32 -0.2 677,733 + HP15_656 0.85 -0.0 677,736 - HP15_656 0.87 -0.3 677,793 + +0.5 677,793 + +1.5 677,865 - +0.6 678,000 + HP15_657 0.15 +0.3 678,040 - HP15_657 0.18 +0.4 678,052 - HP15_657 0.19 +0.3 678,092 + HP15_657 0.22 +0.5 678,130 - HP15_657 0.24 -0.6 678,130 - HP15_657 0.24 -0.2 678,146 - HP15_657 0.25 +1.3 678,201 + HP15_657 0.29 +0.6 678,273 - HP15_657 0.34 -0.0 678,319 - HP15_657 0.37 -0.1 678,333 - HP15_657 0.38 -0.3 678,409 + HP15_657 0.44 -0.3 678,409 - HP15_657 0.44 +1.8 678,466 + HP15_657 0.48 +1.3 678,479 - HP15_657 0.48 -0.5 678,500 + HP15_657 0.50 +0.8 678,505 + HP15_657 0.50 +0.7 678,515 + HP15_657 0.51 +1.2 678,560 - HP15_657 0.54 +0.1 678,624 + HP15_657 0.59 +0.6 678,624 + HP15_657 0.59 +0.2 678,664 + HP15_657 0.61 +0.7 678,664 + HP15_657 0.61 +0.9 678,779 - HP15_657 0.69 +1.0 678,826 + HP15_657 0.73 +1.5 678,853 + HP15_657 0.74 +0.5 678,900 - HP15_657 0.78 +0.5 678,964 + HP15_657 0.82 +1.0 678,995 - HP15_657 0.84 +0.9 679,085 + -1.0 679,107 - -1.0 679,233 + +0.4 679,244 + +1.0 679,312 + +0.0 679,463 + +0.5 679,619 - HP15_658 0.16 +1.7 679,798 - HP15_658 0.23 +0.5 679,825 + HP15_658 0.24 -0.0 679,833 - HP15_658 0.24 +0.7 679,833 - HP15_658 0.24 +1.9 679,833 - HP15_658 0.24 +2.2 679,925 + HP15_658 0.28 +0.2 679,942 + HP15_658 0.28 +0.9 679,942 + HP15_658 0.28 -0.5 679,950 - HP15_658 0.29 +0.8 679,950 - HP15_658 0.29 -0.3 679,965 - HP15_658 0.29 -0.1 680,160 - HP15_658 0.37 +1.6 680,160 - HP15_658 0.37 +1.6 680,166 - HP15_658 0.37 +0.3 680,199 + HP15_658 0.38 -0.1 680,199 + HP15_658 0.38 +0.9 680,199 + HP15_658 0.38 +1.4 680,207 - HP15_658 0.39 +0.1
Or see this region's nucleotide sequence