Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_237 and HP15_238 overlap by 4 nucleotides HP15_238 and HP15_239 are separated by 8 nucleotides HP15_239 and HP15_240 overlap by 20 nucleotides
HP15_237: HP15_237 - HemY domain protein, at 222,307 to 223,548
_237
HP15_238: HP15_238 - protein of unknown function, HemX, at 223,545 to 224,669
_238
HP15_239: HP15_239 - uroporphyrinogen-III synthase, at 224,678 to 225,487
_239
HP15_240: HP15_240 - porphobilinogen deaminase, at 225,468 to 226,409
_240
Position (kb) 223
224
225 Strain fitness (log2 ratio) -1
0
1 at 222.640 kb on - strand, within HP15_237 at 222.651 kb on - strand, within HP15_237 at 222.665 kb on + strand, within HP15_237 at 222.715 kb on - strand, within HP15_237 at 222.777 kb on + strand, within HP15_237 at 222.843 kb on + strand, within HP15_237 at 222.848 kb on + strand, within HP15_237 at 222.920 kb on + strand, within HP15_237 at 223.035 kb on + strand, within HP15_237 at 223.071 kb on + strand, within HP15_237 at 223.103 kb on - strand, within HP15_237 at 223.108 kb on + strand, within HP15_237 at 223.108 kb on + strand, within HP15_237 at 223.108 kb on + strand, within HP15_237 at 223.189 kb on + strand, within HP15_237 at 223.281 kb on - strand, within HP15_237 at 223.426 kb on - strand at 223.464 kb on - strand at 223.472 kb on + strand at 223.497 kb on + strand at 223.500 kb on - strand at 223.640 kb on - strand at 223.700 kb on + strand, within HP15_238 at 223.710 kb on - strand, within HP15_238 at 223.893 kb on - strand, within HP15_238 at 223.898 kb on + strand, within HP15_238 at 223.908 kb on + strand, within HP15_238 at 223.939 kb on - strand, within HP15_238 at 223.939 kb on - strand, within HP15_238 at 223.953 kb on + strand, within HP15_238 at 224.038 kb on - strand, within HP15_238 at 224.040 kb on + strand, within HP15_238 at 224.048 kb on - strand, within HP15_238 at 224.054 kb on - strand, within HP15_238 at 224.523 kb on - strand, within HP15_238
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 222,640 - HP15_237 0.27 -0.2 222,651 - HP15_237 0.28 -1.0 222,665 + HP15_237 0.29 +0.4 222,715 - HP15_237 0.33 +0.2 222,777 + HP15_237 0.38 +0.1 222,843 + HP15_237 0.43 -0.4 222,848 + HP15_237 0.44 +0.1 222,920 + HP15_237 0.49 +0.1 223,035 + HP15_237 0.59 +0.9 223,071 + HP15_237 0.62 +0.1 223,103 - HP15_237 0.64 +0.8 223,108 + HP15_237 0.64 +1.2 223,108 + HP15_237 0.64 +0.7 223,108 + HP15_237 0.64 +0.8 223,189 + HP15_237 0.71 +0.8 223,281 - HP15_237 0.78 +0.6 223,426 - +0.5 223,464 - -0.4 223,472 + +0.0 223,497 + -0.2 223,500 - +1.6 223,640 - +1.0 223,700 + HP15_238 0.14 +0.2 223,710 - HP15_238 0.15 +0.8 223,893 - HP15_238 0.31 -1.5 223,898 + HP15_238 0.31 -0.0 223,908 + HP15_238 0.32 +0.7 223,939 - HP15_238 0.35 -0.1 223,939 - HP15_238 0.35 -0.4 223,953 + HP15_238 0.36 +0.0 224,038 - HP15_238 0.44 +0.7 224,040 + HP15_238 0.44 +0.1 224,048 - HP15_238 0.45 -0.4 224,054 - HP15_238 0.45 +0.4 224,523 - HP15_238 0.87 -0.3
Or see this region's nucleotide sequence