Experiment: L-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_926 and HP15_927 overlap by 4 nucleotides HP15_927 and HP15_928 are separated by 78 nucleotides HP15_928 and HP15_929 are separated by 45 nucleotides HP15_929 and HP15_930 are separated by 2 nucleotides
HP15_926: HP15_926 - branched-chain amino acid ABC transporter, ATP-binding protein, at 940,101 to 940,904
_926
HP15_927: HP15_927 - branched-chain amino acid ABC transporter, ATP-binding protein, at 940,901 to 941,623
_927
HP15_928: HP15_928 - extracellular ligand-binding receptor, at 941,702 to 942,907
_928
HP15_929: HP15_929 - branched-chain amino acid ABC transporter, permease protein, at 942,953 to 943,900
_929
HP15_930: HP15_930 - branched-chain amino acid transport permease, at 943,903 to 944,898
_930
Position (kb)
941
942
943 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 940.709 kb on - strand, within HP15_926 at 940.753 kb on + strand, within HP15_926 at 940.771 kb on - strand, within HP15_926 at 940.772 kb on - strand, within HP15_926 at 940.986 kb on + strand, within HP15_927 at 940.997 kb on + strand, within HP15_927 at 941.005 kb on - strand, within HP15_927 at 941.005 kb on - strand, within HP15_927 at 941.020 kb on - strand, within HP15_927 at 941.053 kb on - strand, within HP15_927 at 941.097 kb on + strand, within HP15_927 at 941.256 kb on + strand, within HP15_927 at 941.288 kb on - strand, within HP15_927 at 941.313 kb on + strand, within HP15_927 at 941.413 kb on + strand, within HP15_927 at 941.441 kb on - strand, within HP15_927 at 941.479 kb on + strand, within HP15_927 at 941.505 kb on + strand, within HP15_927 at 941.537 kb on + strand, within HP15_927 at 941.545 kb on - strand, within HP15_927 at 941.583 kb on + strand at 941.583 kb on + strand at 941.659 kb on + strand at 941.809 kb on + strand at 941.924 kb on - strand, within HP15_928 at 941.970 kb on - strand, within HP15_928 at 942.083 kb on + strand, within HP15_928 at 942.194 kb on + strand, within HP15_928 at 942.264 kb on + strand, within HP15_928 at 942.268 kb on - strand, within HP15_928 at 942.311 kb on + strand, within HP15_928 at 942.311 kb on + strand, within HP15_928 at 942.354 kb on + strand, within HP15_928 at 942.389 kb on + strand, within HP15_928 at 942.395 kb on + strand, within HP15_928 at 942.426 kb on + strand, within HP15_928 at 942.474 kb on - strand, within HP15_928 at 942.511 kb on - strand, within HP15_928 at 942.511 kb on - strand, within HP15_928 at 942.572 kb on - strand, within HP15_928 at 942.617 kb on + strand, within HP15_928 at 942.706 kb on - strand, within HP15_928 at 942.706 kb on - strand, within HP15_928 at 942.733 kb on + strand, within HP15_928 at 942.779 kb on - strand, within HP15_928 at 942.805 kb on + strand at 942.813 kb on - strand at 942.813 kb on - strand at 942.824 kb on - strand at 942.859 kb on + strand at 942.867 kb on - strand at 942.885 kb on + strand at 942.946 kb on - strand at 943.043 kb on + strand at 943.094 kb on + strand, within HP15_929 at 943.094 kb on + strand, within HP15_929 at 943.094 kb on + strand, within HP15_929 at 943.094 kb on + strand, within HP15_929 at 943.102 kb on - strand, within HP15_929 at 943.183 kb on - strand, within HP15_929 at 943.253 kb on + strand, within HP15_929 at 943.309 kb on - strand, within HP15_929 at 943.309 kb on - strand, within HP15_929 at 943.351 kb on - strand, within HP15_929 at 943.389 kb on + strand, within HP15_929 at 943.401 kb on - strand, within HP15_929 at 943.406 kb on - strand, within HP15_929 at 943.414 kb on + strand, within HP15_929 at 943.414 kb on + strand, within HP15_929 at 943.414 kb on + strand, within HP15_929 at 943.422 kb on - strand, within HP15_929 at 943.430 kb on + strand, within HP15_929 at 943.463 kb on + strand, within HP15_929 at 943.470 kb on - strand, within HP15_929 at 943.674 kb on - strand, within HP15_929 at 943.713 kb on - strand, within HP15_929 at 943.744 kb on - strand, within HP15_929 at 943.772 kb on + strand, within HP15_929 at 943.806 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine as (N) remove 940,709 - HP15_926 0.76 -0.7 940,753 + HP15_926 0.81 -0.4 940,771 - HP15_926 0.83 -2.9 940,772 - HP15_926 0.83 -0.5 940,986 + HP15_927 0.12 +1.7 940,997 + HP15_927 0.13 -0.2 941,005 - HP15_927 0.14 +0.8 941,005 - HP15_927 0.14 +2.2 941,020 - HP15_927 0.16 -0.5 941,053 - HP15_927 0.21 -0.7 941,097 + HP15_927 0.27 -0.7 941,256 + HP15_927 0.49 -0.1 941,288 - HP15_927 0.54 -0.4 941,313 + HP15_927 0.57 +0.5 941,413 + HP15_927 0.71 -0.1 941,441 - HP15_927 0.75 -0.4 941,479 + HP15_927 0.80 -0.2 941,505 + HP15_927 0.84 +1.6 941,537 + HP15_927 0.88 -0.3 941,545 - HP15_927 0.89 -0.4 941,583 + +1.7 941,583 + +0.8 941,659 + +0.1 941,809 + -0.2 941,924 - HP15_928 0.18 -0.8 941,970 - HP15_928 0.22 +2.3 942,083 + HP15_928 0.32 +0.2 942,194 + HP15_928 0.41 +1.5 942,264 + HP15_928 0.47 -0.4 942,268 - HP15_928 0.47 -0.0 942,311 + HP15_928 0.50 -0.4 942,311 + HP15_928 0.50 +0.1 942,354 + HP15_928 0.54 -0.4 942,389 + HP15_928 0.57 +2.7 942,395 + HP15_928 0.57 -0.5 942,426 + HP15_928 0.60 +1.4 942,474 - HP15_928 0.64 +1.4 942,511 - HP15_928 0.67 +1.5 942,511 - HP15_928 0.67 -0.3 942,572 - HP15_928 0.72 +1.3 942,617 + HP15_928 0.76 -0.3 942,706 - HP15_928 0.83 +0.1 942,706 - HP15_928 0.83 +1.4 942,733 + HP15_928 0.85 +2.0 942,779 - HP15_928 0.89 +0.0 942,805 + +0.0 942,813 - -0.5 942,813 - +1.7 942,824 - -0.3 942,859 + -0.7 942,867 - +0.1 942,885 + -0.6 942,946 - -0.4 943,043 + +0.2 943,094 + HP15_929 0.15 +1.7 943,094 + HP15_929 0.15 -1.3 943,094 + HP15_929 0.15 -0.2 943,094 + HP15_929 0.15 +1.9 943,102 - HP15_929 0.16 -2.0 943,183 - HP15_929 0.24 +2.1 943,253 + HP15_929 0.32 +0.2 943,309 - HP15_929 0.38 -0.0 943,309 - HP15_929 0.38 +1.2 943,351 - HP15_929 0.42 +1.8 943,389 + HP15_929 0.46 +0.6 943,401 - HP15_929 0.47 +0.1 943,406 - HP15_929 0.48 +1.6 943,414 + HP15_929 0.49 +0.1 943,414 + HP15_929 0.49 -1.0 943,414 + HP15_929 0.49 -0.2 943,422 - HP15_929 0.49 +1.7 943,430 + HP15_929 0.50 -0.4 943,463 + HP15_929 0.54 -1.3 943,470 - HP15_929 0.55 -1.0 943,674 - HP15_929 0.76 -0.5 943,713 - HP15_929 0.80 +0.4 943,744 - HP15_929 0.83 -0.6 943,772 + HP15_929 0.86 -0.8 943,806 + +2.1
Or see this region's nucleotide sequence