Experiment: L-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_301 and HP15_302 are separated by 21 nucleotides HP15_302 and HP15_303 are separated by 148 nucleotides HP15_303 and HP15_304 are separated by 86 nucleotides HP15_304 and HP15_305 are separated by 172 nucleotides
HP15_301: HP15_301 - conserved hypothetical protein, at 291,999 to 292,865
_301
HP15_302: HP15_302 - protein containing pseudouridine synthase domain, at 292,887 to 293,708
_302
HP15_303: HP15_303 - guanylate kinase, at 293,857 to 294,489
_303
HP15_304: HP15_304 - DNA-directed RNA polymerase subunit omega, at 294,576 to 294,788
_304
HP15_305: HP15_305 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 294,961 to 297,102
_305
Position (kb) 293
294
295 Strain fitness (log2 ratio) -2
-1
0
1
2 at 292.903 kb on - strand at 292.939 kb on + strand at 292.944 kb on + strand at 292.944 kb on + strand at 292.944 kb on + strand at 292.952 kb on - strand at 292.983 kb on + strand, within HP15_302 at 293.116 kb on + strand, within HP15_302 at 293.134 kb on - strand, within HP15_302 at 293.233 kb on + strand, within HP15_302 at 293.266 kb on - strand, within HP15_302 at 293.317 kb on + strand, within HP15_302 at 293.425 kb on + strand, within HP15_302 at 293.440 kb on + strand, within HP15_302 at 293.440 kb on + strand, within HP15_302 at 293.448 kb on - strand, within HP15_302 at 293.448 kb on - strand, within HP15_302 at 293.553 kb on + strand, within HP15_302 at 293.664 kb on + strand at 293.678 kb on + strand at 293.734 kb on - strand at 293.745 kb on - strand at 293.759 kb on + strand at 293.759 kb on + strand at 293.759 kb on + strand at 293.760 kb on - strand at 293.767 kb on - strand at 293.769 kb on - strand at 293.769 kb on - strand at 293.838 kb on + strand at 293.838 kb on + strand at 294.764 kb on + strand, within HP15_304
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine as (N) remove 292,903 - -0.5 292,939 + -0.1 292,944 + -0.4 292,944 + -0.6 292,944 + -0.6 292,952 - +1.9 292,983 + HP15_302 0.12 +0.1 293,116 + HP15_302 0.28 +0.0 293,134 - HP15_302 0.30 +1.9 293,233 + HP15_302 0.42 +1.8 293,266 - HP15_302 0.46 -0.3 293,317 + HP15_302 0.52 -0.4 293,425 + HP15_302 0.65 +0.1 293,440 + HP15_302 0.67 +1.4 293,440 + HP15_302 0.67 +1.0 293,448 - HP15_302 0.68 -0.1 293,448 - HP15_302 0.68 +0.8 293,553 + HP15_302 0.81 +0.7 293,664 + +0.6 293,678 + +0.8 293,734 - +0.7 293,745 - -1.1 293,759 + +2.3 293,759 + -0.3 293,759 + -1.3 293,760 - -0.4 293,767 - +2.1 293,769 - -0.1 293,769 - -0.1 293,838 + -2.1 293,838 + +2.0 294,764 + HP15_304 0.88 -0.3
Or see this region's nucleotide sequence