Experiment: L-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_11 and HP15_12 are separated by 1 nucleotides HP15_12 and HP15_13 are separated by 143 nucleotides HP15_13 and HP15_14 are separated by 38 nucleotides HP15_14 and HP15_15 are separated by 319 nucleotides
HP15_11: HP15_11 - conserved hypothetical protein, at 13,976 to 14,941
_11
HP15_12: HP15_12 - enoyl-CoA hydratase/isomerase, at 14,943 to 15,755
_12
HP15_13: HP15_13 - short-chain dehydrogenase/reductase SDR, at 15,899 to 16,627
_13
HP15_14: HP15_14 - transcriptional regulator, TetR family, at 16,666 to 17,247
_14
HP15_15: HP15_15 - short chain dehydrogenase/reductase, at 17,567 to 18,214
_15
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 14.903 kb on - strand at 14.918 kb on + strand at 14.920 kb on + strand at 14.935 kb on + strand at 14.940 kb on + strand at 15.103 kb on - strand, within HP15_12 at 15.157 kb on - strand, within HP15_12 at 15.235 kb on + strand, within HP15_12 at 15.235 kb on + strand, within HP15_12 at 15.312 kb on - strand, within HP15_12 at 15.315 kb on + strand, within HP15_12 at 15.315 kb on + strand, within HP15_12 at 15.354 kb on + strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.485 kb on + strand, within HP15_12 at 15.485 kb on + strand, within HP15_12 at 15.485 kb on + strand, within HP15_12 at 15.493 kb on + strand, within HP15_12 at 15.493 kb on + strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.501 kb on - strand, within HP15_12 at 15.546 kb on + strand, within HP15_12 at 15.839 kb on + strand at 15.851 kb on + strand at 16.008 kb on + strand, within HP15_13 at 16.015 kb on + strand, within HP15_13 at 16.069 kb on - strand, within HP15_13 at 16.079 kb on - strand, within HP15_13 at 16.086 kb on + strand, within HP15_13 at 16.100 kb on - strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.162 kb on + strand, within HP15_13 at 16.170 kb on - strand, within HP15_13 at 16.182 kb on + strand, within HP15_13 at 16.183 kb on - strand, within HP15_13 at 16.183 kb on - strand, within HP15_13 at 16.207 kb on + strand, within HP15_13 at 16.242 kb on + strand, within HP15_13 at 16.247 kb on + strand, within HP15_13 at 16.250 kb on - strand, within HP15_13 at 16.250 kb on - strand, within HP15_13 at 16.280 kb on + strand, within HP15_13 at 16.291 kb on + strand, within HP15_13 at 16.291 kb on + strand, within HP15_13 at 16.291 kb on + strand, within HP15_13 at 16.299 kb on - strand, within HP15_13 at 16.404 kb on + strand, within HP15_13 at 16.412 kb on - strand, within HP15_13 at 16.413 kb on + strand, within HP15_13 at 16.413 kb on + strand, within HP15_13 at 16.421 kb on - strand, within HP15_13 at 16.421 kb on - strand, within HP15_13 at 16.421 kb on - strand, within HP15_13 at 16.460 kb on - strand, within HP15_13 at 16.504 kb on - strand, within HP15_13 at 16.563 kb on + strand at 16.567 kb on + strand at 16.571 kb on - strand at 16.571 kb on - strand at 16.606 kb on + strand at 16.614 kb on - strand at 16.614 kb on - strand at 16.619 kb on + strand at 16.620 kb on + strand at 16.627 kb on - strand at 16.629 kb on - strand at 16.714 kb on - strand at 16.729 kb on - strand, within HP15_14 at 16.729 kb on - strand, within HP15_14 at 16.731 kb on + strand, within HP15_14 at 16.731 kb on + strand, within HP15_14 at 16.734 kb on - strand, within HP15_14 at 16.795 kb on + strand, within HP15_14 at 16.795 kb on + strand, within HP15_14 at 17.048 kb on - strand, within HP15_14 at 17.070 kb on + strand, within HP15_14 at 17.089 kb on - strand, within HP15_14 at 17.092 kb on + strand, within HP15_14 at 17.100 kb on - strand, within HP15_14 at 17.102 kb on + strand, within HP15_14 at 17.227 kb on + strand at 17.227 kb on + strand at 17.391 kb on - strand at 17.455 kb on + strand at 17.460 kb on + strand at 17.486 kb on + strand at 17.486 kb on + strand at 17.494 kb on - strand at 17.494 kb on - strand at 17.505 kb on + strand at 17.519 kb on + strand at 17.627 kb on + strand at 17.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine as (N) remove 14,903 - +2.3 14,918 + +0.3 14,920 + -0.4 14,935 + -0.7 14,940 + +0.6 15,103 - HP15_12 0.20 -0.7 15,157 - HP15_12 0.26 -0.6 15,235 + HP15_12 0.36 -1.1 15,235 + HP15_12 0.36 -0.3 15,312 - HP15_12 0.45 -0.7 15,315 + HP15_12 0.46 -0.2 15,315 + HP15_12 0.46 +1.5 15,354 + HP15_12 0.51 -1.2 15,463 - HP15_12 0.64 +0.4 15,463 - HP15_12 0.64 -0.2 15,463 - HP15_12 0.64 -0.8 15,463 - HP15_12 0.64 -1.3 15,485 + HP15_12 0.67 +1.8 15,485 + HP15_12 0.67 -0.2 15,485 + HP15_12 0.67 -0.0 15,493 + HP15_12 0.68 +1.8 15,493 + HP15_12 0.68 +2.2 15,493 - HP15_12 0.68 +1.9 15,493 - HP15_12 0.68 +1.5 15,493 - HP15_12 0.68 +1.9 15,493 - HP15_12 0.68 +1.7 15,501 - HP15_12 0.69 +1.4 15,546 + HP15_12 0.74 -0.1 15,839 + +1.6 15,851 + -1.1 16,008 + HP15_13 0.15 -0.2 16,015 + HP15_13 0.16 +1.3 16,069 - HP15_13 0.23 -0.2 16,079 - HP15_13 0.25 -1.3 16,086 + HP15_13 0.26 -0.7 16,100 - HP15_13 0.28 +1.6 16,162 + HP15_13 0.36 +2.1 16,162 + HP15_13 0.36 +1.6 16,162 + HP15_13 0.36 -0.3 16,162 + HP15_13 0.36 +1.9 16,170 - HP15_13 0.37 -0.9 16,182 + HP15_13 0.39 +1.7 16,183 - HP15_13 0.39 -1.0 16,183 - HP15_13 0.39 -1.1 16,207 + HP15_13 0.42 +2.1 16,242 + HP15_13 0.47 -0.6 16,247 + HP15_13 0.48 -0.1 16,250 - HP15_13 0.48 -1.1 16,250 - HP15_13 0.48 +1.7 16,280 + HP15_13 0.52 -0.4 16,291 + HP15_13 0.54 +2.3 16,291 + HP15_13 0.54 -0.0 16,291 + HP15_13 0.54 -1.6 16,299 - HP15_13 0.55 +1.7 16,404 + HP15_13 0.69 +1.5 16,412 - HP15_13 0.70 -1.7 16,413 + HP15_13 0.71 -0.8 16,413 + HP15_13 0.71 +1.4 16,421 - HP15_13 0.72 +1.5 16,421 - HP15_13 0.72 -0.7 16,421 - HP15_13 0.72 +1.8 16,460 - HP15_13 0.77 +2.1 16,504 - HP15_13 0.83 -0.6 16,563 + +1.9 16,567 + -0.3 16,571 - -0.6 16,571 - -0.3 16,606 + +1.7 16,614 - +1.6 16,614 - +1.1 16,619 + +1.6 16,620 + -0.5 16,627 - +2.1 16,629 - +1.7 16,714 - -1.0 16,729 - HP15_14 0.11 +0.2 16,729 - HP15_14 0.11 -0.8 16,731 + HP15_14 0.11 -0.6 16,731 + HP15_14 0.11 +1.3 16,734 - HP15_14 0.12 -1.7 16,795 + HP15_14 0.22 -0.9 16,795 + HP15_14 0.22 +2.0 17,048 - HP15_14 0.66 -0.8 17,070 + HP15_14 0.69 -1.5 17,089 - HP15_14 0.73 -0.3 17,092 + HP15_14 0.73 -0.4 17,100 - HP15_14 0.75 -1.0 17,102 + HP15_14 0.75 -2.0 17,227 + -1.1 17,227 + -2.0 17,391 - -0.3 17,455 + -0.1 17,460 + -0.1 17,486 + -0.3 17,486 + +1.1 17,494 - +1.8 17,494 - -0.1 17,505 + -0.4 17,519 + +1.6 17,627 + +0.0 17,627 + +1.5
Or see this region's nucleotide sequence