Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1362 and Shew_1363 are separated by 155 nucleotides Shew_1363 and Shew_1364 overlap by 8 nucleotides
Shew_1362: Shew_1362 - sigma-54 dependent trancsriptional regulator (RefSeq), at 1,570,438 to 1,571,871
_1362
Shew_1363: Shew_1363 - PAS/PAC sensor signal transduction histidine kinase (RefSeq), at 1,572,027 to 1,573,115
_1363
Shew_1364: Shew_1364 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 1,573,108 to 1,574,451
_1364
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1571.464 kb on + strand, within Shew_1362 at 1572.047 kb on + strand at 1572.098 kb on - strand at 1572.261 kb on - strand, within Shew_1363 at 1572.350 kb on + strand, within Shew_1363 at 1572.509 kb on - strand, within Shew_1363 at 1572.529 kb on + strand, within Shew_1363 at 1572.529 kb on + strand, within Shew_1363 at 1572.529 kb on + strand, within Shew_1363 at 1572.969 kb on + strand, within Shew_1363 at 1572.969 kb on + strand, within Shew_1363 at 1572.969 kb on + strand, within Shew_1363 at 1572.977 kb on - strand, within Shew_1363 at 1573.016 kb on - strand at 1573.518 kb on + strand, within Shew_1364 at 1573.827 kb on + strand, within Shew_1364 at 1573.886 kb on - strand, within Shew_1364
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,571,464 + Shew_1362 0.72 +6.7 1,572,047 + +4.8 1,572,098 - +4.6 1,572,261 - Shew_1363 0.21 +4.2 1,572,350 + Shew_1363 0.30 +4.2 1,572,509 - Shew_1363 0.44 +4.4 1,572,529 + Shew_1363 0.46 +4.1 1,572,529 + Shew_1363 0.46 +4.1 1,572,529 + Shew_1363 0.46 +5.0 1,572,969 + Shew_1363 0.87 +4.0 1,572,969 + Shew_1363 0.87 +3.9 1,572,969 + Shew_1363 0.87 +4.1 1,572,977 - Shew_1363 0.87 +4.1 1,573,016 - +4.5 1,573,518 + Shew_1364 0.31 +4.2 1,573,827 + Shew_1364 0.53 +4.4 1,573,886 - Shew_1364 0.58 +4.0
Or see this region's nucleotide sequence