Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1119 and Shew_R0024 are separated by 186 nucleotides Shew_R0024 and Shew_R0025 are separated by 7 nucleotides Shew_R0025 and Shew_1120 are separated by 386 nucleotides Shew_1120 and Shew_1121 are separated by 53 nucleotides Shew_1121 and Shew_1122 are separated by 76 nucleotides Shew_1122 and Shew_1123 are separated by 2 nucleotides
Shew_1119: Shew_1119 - glucose/galactose transporter (RefSeq), at 1,294,566 to 1,295,822
_1119
Shew_R0024: Shew_R0024 - tRNA-Thr (RefSeq), at 1,296,009 to 1,296,084
_R0024
Shew_R0025: Shew_R0025 - tRNA-Phe (RefSeq), at 1,296,092 to 1,296,167
_R0025
Shew_1120: Shew_1120 - cytochrome B561 (RefSeq), at 1,296,554 to 1,297,108
_1120
Shew_1121: Shew_1121 - hypothetical protein (RefSeq), at 1,297,162 to 1,297,695
_1121
Shew_1122: Shew_1122 - hypothetical protein (RefSeq), at 1,297,772 to 1,298,050
_1122
Shew_1123: Shew_1123 - A/G-specific adenine glycosylase (RefSeq), at 1,298,053 to 1,299,159
_1123
Position (kb) 1296
1297
1298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1295.790 kb on + strand at 1295.805 kb on - strand at 1295.812 kb on + strand at 1296.731 kb on + strand, within Shew_1120 at 1296.731 kb on + strand, within Shew_1120 at 1296.731 kb on + strand, within Shew_1120 at 1296.739 kb on - strand, within Shew_1120 at 1296.831 kb on + strand, within Shew_1120 at 1297.037 kb on - strand, within Shew_1120 at 1297.170 kb on + strand at 1297.249 kb on + strand, within Shew_1121 at 1297.406 kb on + strand, within Shew_1121 at 1297.436 kb on + strand, within Shew_1121 at 1297.814 kb on - strand, within Shew_1122 at 1297.983 kb on + strand, within Shew_1122
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,295,790 + +0.1 1,295,805 - -0.3 1,295,812 + +1.6 1,296,731 + Shew_1120 0.32 +0.2 1,296,731 + Shew_1120 0.32 -0.5 1,296,731 + Shew_1120 0.32 +0.3 1,296,739 - Shew_1120 0.33 +2.1 1,296,831 + Shew_1120 0.50 +0.9 1,297,037 - Shew_1120 0.87 -0.2 1,297,170 + -0.5 1,297,249 + Shew_1121 0.16 -0.2 1,297,406 + Shew_1121 0.46 -0.3 1,297,436 + Shew_1121 0.51 +1.2 1,297,814 - Shew_1122 0.15 -1.3 1,297,983 + Shew_1122 0.76 -2.8
Or see this region's nucleotide sequence