Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0843 and nrfA are separated by 247 nucleotides nrfA and Shew_0845 are separated by 74 nucleotides Shew_0845 and Shew_0846 are separated by 252 nucleotides
Shew_0843: Shew_0843 - nitrate/nitrite sensor protein NarQ (RefSeq), at 965,163 to 966,866
_0843
Shew_0844: nrfA - cytochrome c nitrite reductase (RefSeq), at 967,114 to 968,514
nrfA
Shew_0845: Shew_0845 - isochorismatase hydrolase (RefSeq), at 968,589 to 969,131
_0845
Shew_0846: Shew_0846 - nitrogen regulatory protein P-II (RefSeq), at 969,384 to 969,722
_0846
Position (kb)
967
968
969 Strain fitness (log2 ratio)
-1
0
1
2 at 966.200 kb on - strand, within Shew_0843 at 966.292 kb on + strand, within Shew_0843 at 966.571 kb on - strand, within Shew_0843 at 967.147 kb on - strand at 967.202 kb on + strand at 967.210 kb on - strand at 967.256 kb on - strand, within nrfA at 967.270 kb on + strand, within nrfA at 967.341 kb on - strand, within nrfA at 967.395 kb on + strand, within nrfA at 967.480 kb on - strand, within nrfA at 967.498 kb on + strand, within nrfA at 967.541 kb on - strand, within nrfA at 967.579 kb on - strand, within nrfA at 967.595 kb on + strand, within nrfA at 967.686 kb on + strand, within nrfA at 967.721 kb on + strand, within nrfA at 967.791 kb on + strand, within nrfA at 967.911 kb on + strand, within nrfA at 968.054 kb on + strand, within nrfA at 968.141 kb on - strand, within nrfA at 968.361 kb on + strand, within nrfA at 968.361 kb on + strand, within nrfA at 968.524 kb on + strand at 968.634 kb on - strand at 968.762 kb on + strand, within Shew_0845 at 968.981 kb on + strand, within Shew_0845 at 969.098 kb on - strand at 969.379 kb on - strand at 969.447 kb on + strand, within Shew_0846 at 969.505 kb on + strand, within Shew_0846
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 966,200 - Shew_0843 0.61 +0.4 966,292 + Shew_0843 0.66 +1.2 966,571 - Shew_0843 0.83 +0.1 967,147 - -0.1 967,202 + +1.6 967,210 - +0.7 967,256 - nrfA Shew_0844 0.10 +0.5 967,270 + nrfA Shew_0844 0.11 +0.1 967,341 - nrfA Shew_0844 0.16 +0.7 967,395 + nrfA Shew_0844 0.20 +0.2 967,480 - nrfA Shew_0844 0.26 +0.2 967,498 + nrfA Shew_0844 0.27 +0.6 967,541 - nrfA Shew_0844 0.30 -0.4 967,579 - nrfA Shew_0844 0.33 +1.9 967,595 + nrfA Shew_0844 0.34 +0.5 967,686 + nrfA Shew_0844 0.41 +0.5 967,721 + nrfA Shew_0844 0.43 -0.4 967,791 + nrfA Shew_0844 0.48 -0.6 967,911 + nrfA Shew_0844 0.57 +0.2 968,054 + nrfA Shew_0844 0.67 -0.5 968,141 - nrfA Shew_0844 0.73 +1.2 968,361 + nrfA Shew_0844 0.89 +0.5 968,361 + nrfA Shew_0844 0.89 +0.6 968,524 + +0.4 968,634 - +1.3 968,762 + Shew_0845 0.32 +0.2 968,981 + Shew_0845 0.72 +0.2 969,098 - -0.0 969,379 - +1.0 969,447 + Shew_0846 0.19 -0.0 969,505 + Shew_0846 0.36 +0.4
Or see this region's nucleotide sequence