Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argC and Shew_0203 are separated by 28 nucleotides Shew_0203 and Shew_0204 are separated by 42 nucleotides
Shew_0202: argC - N-acetyl-gamma-glutamyl-phosphate reductase (RefSeq), at 215,298 to 216,278
argC
Shew_0203: Shew_0203 - acetylglutamate kinase (RefSeq), at 216,307 to 217,092
_0203
Shew_0204: Shew_0204 - ornithine carbamoyltransferase (RefSeq), at 217,135 to 218,040
_0204
Position (kb) 216
217
218 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 215.553 kb on + strand, within argC at 215.606 kb on + strand, within argC at 215.772 kb on + strand, within argC at 216.029 kb on + strand, within argC at 216.037 kb on - strand, within argC at 216.037 kb on - strand, within argC at 216.075 kb on - strand, within argC at 216.098 kb on - strand, within argC at 216.145 kb on + strand, within argC at 216.358 kb on - strand at 216.360 kb on + strand at 216.465 kb on + strand, within Shew_0203 at 216.473 kb on - strand, within Shew_0203 at 216.495 kb on + strand, within Shew_0203 at 216.511 kb on - strand, within Shew_0203 at 216.706 kb on + strand, within Shew_0203 at 216.786 kb on + strand, within Shew_0203 at 216.865 kb on + strand, within Shew_0203 at 216.908 kb on - strand, within Shew_0203 at 216.914 kb on + strand, within Shew_0203 at 216.932 kb on - strand, within Shew_0203 at 216.957 kb on + strand, within Shew_0203 at 216.957 kb on + strand, within Shew_0203 at 216.963 kb on + strand, within Shew_0203 at 216.973 kb on + strand, within Shew_0203 at 217.077 kb on + strand at 217.263 kb on - strand, within Shew_0204 at 217.373 kb on - strand, within Shew_0204 at 217.517 kb on - strand, within Shew_0204 at 217.826 kb on + strand, within Shew_0204
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 215,553 + argC Shew_0202 0.26 -5.9 215,606 + argC Shew_0202 0.31 -5.8 215,772 + argC Shew_0202 0.48 -5.2 216,029 + argC Shew_0202 0.75 -4.7 216,037 - argC Shew_0202 0.75 -3.9 216,037 - argC Shew_0202 0.75 -4.6 216,075 - argC Shew_0202 0.79 -6.5 216,098 - argC Shew_0202 0.82 -3.2 216,145 + argC Shew_0202 0.86 -4.8 216,358 - -5.2 216,360 + -5.5 216,465 + Shew_0203 0.20 -5.0 216,473 - Shew_0203 0.21 -3.9 216,495 + Shew_0203 0.24 -5.6 216,511 - Shew_0203 0.26 -3.5 216,706 + Shew_0203 0.51 -1.5 216,786 + Shew_0203 0.61 -4.3 216,865 + Shew_0203 0.71 -5.4 216,908 - Shew_0203 0.76 -2.4 216,914 + Shew_0203 0.77 -6.1 216,932 - Shew_0203 0.80 -3.9 216,957 + Shew_0203 0.83 -2.7 216,957 + Shew_0203 0.83 -3.6 216,963 + Shew_0203 0.83 -4.7 216,973 + Shew_0203 0.85 -3.5 217,077 + +0.1 217,263 - Shew_0204 0.14 -6.6 217,373 - Shew_0204 0.26 -2.0 217,517 - Shew_0204 0.42 -3.5 217,826 + Shew_0204 0.76 -3.0
Or see this region's nucleotide sequence