Experiment: m.b. Tetracycline 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2210 and Dshi_2211 are separated by 102 nucleotides Dshi_2211 and Dshi_2212 are separated by 9 nucleotides Dshi_2212 and Dshi_2213 are separated by 96 nucleotides Dshi_2213 and Dshi_2214 are separated by 48 nucleotides Dshi_2214 and Dshi_2215 are separated by 9 nucleotides
Dshi_2210: Dshi_2210 - hypothetical protein (RefSeq), at 2,336,408 to 2,338,699
_2210
Dshi_2211: Dshi_2211 - protein of unknown function DUF427 (RefSeq), at 2,338,802 to 2,339,278
_2211
Dshi_2212: Dshi_2212 - pyridoxamine 5'-phosphate oxidase-related FMN-binding (RefSeq), at 2,339,288 to 2,339,782
_2212
Dshi_2213: Dshi_2213 - UspA domain protein (RefSeq), at 2,339,879 to 2,340,286
_2213
Dshi_2214: Dshi_2214 - Antibiotic biosynthesis monooxygenase (RefSeq), at 2,340,335 to 2,340,664
_2214
Dshi_2215: Dshi_2215 - hypothetical protein (RefSeq), at 2,340,674 to 2,341,336
_2215
Position (kb)
2339
2340 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2338.327 kb on + strand, within Dshi_2210 at 2338.335 kb on - strand, within Dshi_2210 at 2338.361 kb on + strand, within Dshi_2210 at 2338.376 kb on + strand, within Dshi_2210 at 2338.386 kb on + strand, within Dshi_2210 at 2338.442 kb on - strand, within Dshi_2210 at 2338.456 kb on + strand, within Dshi_2210 at 2338.459 kb on - strand, within Dshi_2210 at 2338.641 kb on + strand at 2338.649 kb on - strand at 2338.664 kb on + strand at 2338.724 kb on - strand at 2338.755 kb on - strand at 2338.755 kb on - strand at 2338.788 kb on + strand at 2338.836 kb on + strand at 2338.860 kb on + strand, within Dshi_2211 at 2338.951 kb on + strand, within Dshi_2211 at 2338.953 kb on + strand, within Dshi_2211 at 2338.960 kb on + strand, within Dshi_2211 at 2338.991 kb on - strand, within Dshi_2211 at 2339.041 kb on + strand, within Dshi_2211 at 2339.136 kb on - strand, within Dshi_2211 at 2339.158 kb on - strand, within Dshi_2211 at 2339.182 kb on - strand at 2339.325 kb on + strand at 2339.361 kb on + strand, within Dshi_2212 at 2339.403 kb on + strand, within Dshi_2212 at 2339.407 kb on + strand, within Dshi_2212 at 2339.410 kb on - strand, within Dshi_2212 at 2339.432 kb on + strand, within Dshi_2212 at 2339.443 kb on + strand, within Dshi_2212 at 2339.451 kb on - strand, within Dshi_2212 at 2339.474 kb on + strand, within Dshi_2212 at 2339.570 kb on - strand, within Dshi_2212 at 2339.759 kb on + strand at 2339.763 kb on + strand at 2339.763 kb on + strand at 2339.832 kb on + strand at 2339.840 kb on - strand at 2339.858 kb on + strand at 2339.858 kb on + strand at 2339.874 kb on + strand at 2339.877 kb on - strand at 2339.942 kb on + strand, within Dshi_2213 at 2339.962 kb on + strand, within Dshi_2213 at 2340.081 kb on + strand, within Dshi_2213 at 2340.081 kb on + strand, within Dshi_2213 at 2340.166 kb on + strand, within Dshi_2213 at 2340.166 kb on - strand, within Dshi_2213 at 2340.272 kb on + strand at 2340.312 kb on - strand at 2340.405 kb on + strand, within Dshi_2214 at 2340.413 kb on - strand, within Dshi_2214 at 2340.472 kb on - strand, within Dshi_2214 at 2340.497 kb on + strand, within Dshi_2214 at 2340.505 kb on - strand, within Dshi_2214 at 2340.564 kb on + strand, within Dshi_2214 at 2340.612 kb on + strand, within Dshi_2214 at 2340.672 kb on + strand at 2340.696 kb on + strand at 2340.704 kb on - strand at 2340.777 kb on + strand, within Dshi_2215 at 2340.780 kb on - strand, within Dshi_2215
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Tetracycline 0.006 mg/ml remove 2,338,327 + Dshi_2210 0.84 +1.2 2,338,335 - Dshi_2210 0.84 -1.5 2,338,361 + Dshi_2210 0.85 +0.1 2,338,376 + Dshi_2210 0.86 -2.9 2,338,386 + Dshi_2210 0.86 +0.2 2,338,442 - Dshi_2210 0.89 +1.2 2,338,456 + Dshi_2210 0.89 -0.8 2,338,459 - Dshi_2210 0.89 -1.0 2,338,641 + +1.8 2,338,649 - +0.2 2,338,664 + -0.5 2,338,724 - -2.8 2,338,755 - -2.9 2,338,755 - +0.2 2,338,788 + -1.9 2,338,836 + -0.8 2,338,860 + Dshi_2211 0.12 -1.8 2,338,951 + Dshi_2211 0.31 -0.4 2,338,953 + Dshi_2211 0.32 -0.4 2,338,960 + Dshi_2211 0.33 -0.3 2,338,991 - Dshi_2211 0.40 -1.8 2,339,041 + Dshi_2211 0.50 -2.0 2,339,136 - Dshi_2211 0.70 -2.8 2,339,158 - Dshi_2211 0.75 +0.2 2,339,182 - +2.6 2,339,325 + +3.4 2,339,361 + Dshi_2212 0.15 +0.2 2,339,403 + Dshi_2212 0.23 -0.9 2,339,407 + Dshi_2212 0.24 +0.2 2,339,410 - Dshi_2212 0.25 -1.9 2,339,432 + Dshi_2212 0.29 -1.1 2,339,443 + Dshi_2212 0.31 -2.1 2,339,451 - Dshi_2212 0.33 -0.8 2,339,474 + Dshi_2212 0.38 +0.2 2,339,570 - Dshi_2212 0.57 -0.8 2,339,759 + -0.4 2,339,763 + +0.2 2,339,763 + -3.1 2,339,832 + +2.6 2,339,840 - +1.1 2,339,858 + -2.5 2,339,858 + +0.2 2,339,874 + -0.1 2,339,877 - +0.2 2,339,942 + Dshi_2213 0.15 -0.8 2,339,962 + Dshi_2213 0.20 -3.1 2,340,081 + Dshi_2213 0.50 -3.2 2,340,081 + Dshi_2213 0.50 +0.1 2,340,166 + Dshi_2213 0.70 -2.3 2,340,166 - Dshi_2213 0.70 -0.2 2,340,272 + -0.8 2,340,312 - -0.3 2,340,405 + Dshi_2214 0.21 -3.6 2,340,413 - Dshi_2214 0.24 +0.2 2,340,472 - Dshi_2214 0.42 -1.1 2,340,497 + Dshi_2214 0.49 +1.8 2,340,505 - Dshi_2214 0.52 -2.8 2,340,564 + Dshi_2214 0.69 -2.0 2,340,612 + Dshi_2214 0.84 +0.2 2,340,672 + -1.2 2,340,696 + -1.5 2,340,704 - -3.3 2,340,777 + Dshi_2215 0.16 -3.5 2,340,780 - Dshi_2215 0.16 -3.3
Or see this region's nucleotide sequence