Experiment: m.b. Tetracycline 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2092 and Dshi_2093 are separated by 15 nucleotides Dshi_2093 and Dshi_2094 are separated by 330 nucleotides Dshi_2094 and Dshi_2095 are separated by 135 nucleotides Dshi_2095 and Dshi_2096 are separated by 165 nucleotides
Dshi_2092: Dshi_2092 - glycine cleavage T protein (aminomethyl transferase) (RefSeq), at 2,216,299 to 2,217,084
_2092
Dshi_2093: Dshi_2093 - hypothetical protein (RefSeq), at 2,217,100 to 2,217,546
_2093
Dshi_2094: Dshi_2094 - translation elongation factor P (RefSeq), at 2,217,877 to 2,218,518
_2094
Dshi_2095: Dshi_2095 - hypothetical protein (RefSeq), at 2,218,654 to 2,218,974
_2095
Dshi_2096: Dshi_2096 - cobyric acid synthase CobQ (RefSeq), at 2,219,140 to 2,220,600
_2096
Position (kb)
2217
2218
2219 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2216.891 kb on - strand, within Dshi_2092 at 2217.301 kb on + strand, within Dshi_2093 at 2217.328 kb on - strand, within Dshi_2093 at 2217.371 kb on + strand, within Dshi_2093 at 2217.425 kb on - strand, within Dshi_2093 at 2217.499 kb on - strand, within Dshi_2093 at 2217.575 kb on + strand at 2217.735 kb on - strand at 2217.798 kb on + strand at 2217.890 kb on + strand at 2217.922 kb on + strand at 2217.931 kb on - strand at 2217.958 kb on - strand, within Dshi_2094 at 2217.975 kb on - strand, within Dshi_2094 at 2218.018 kb on - strand, within Dshi_2094 at 2218.346 kb on + strand, within Dshi_2094 at 2218.354 kb on - strand, within Dshi_2094 at 2218.490 kb on - strand at 2218.515 kb on + strand at 2218.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Tetracycline 0.006 mg/ml remove 2,216,891 - Dshi_2092 0.75 -1.0 2,217,301 + Dshi_2093 0.45 -0.8 2,217,328 - Dshi_2093 0.51 -2.8 2,217,371 + Dshi_2093 0.61 -2.6 2,217,425 - Dshi_2093 0.73 -1.2 2,217,499 - Dshi_2093 0.89 +1.0 2,217,575 + -0.3 2,217,735 - -0.2 2,217,798 + -2.4 2,217,890 + +0.0 2,217,922 + -1.2 2,217,931 - -0.6 2,217,958 - Dshi_2094 0.13 +1.0 2,217,975 - Dshi_2094 0.15 +0.5 2,218,018 - Dshi_2094 0.22 +0.4 2,218,346 + Dshi_2094 0.73 +1.4 2,218,354 - Dshi_2094 0.74 +0.7 2,218,490 - +2.3 2,218,515 + -2.7 2,218,973 + +2.7
Or see this region's nucleotide sequence