Experiment: m.b. Tetracycline 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0181 and Dshi_0182 are separated by 104 nucleotides Dshi_0182 and Dshi_0183 overlap by 8 nucleotides Dshi_0183 and Dshi_0184 overlap by 4 nucleotides Dshi_0184 and Dshi_0185 are separated by 39 nucleotides
Dshi_0181: Dshi_0181 - methionyl-tRNA formyltransferase (RefSeq), at 184,186 to 185,085
_0181
Dshi_0182: Dshi_0182 - ribonuclease H (RefSeq), at 185,190 to 185,663
_0182
Dshi_0183: Dshi_0183 - Methyltransferase type 11 (RefSeq), at 185,656 to 186,255
_0183
Dshi_0184: Dshi_0184 - hypothetical protein (RefSeq), at 186,252 to 186,704
_0184
Dshi_0185: Dshi_0185 - phosphatidate cytidylyltransferase (RefSeq), at 186,744 to 187,694
_0185
Position (kb) 185
186
187 Strain fitness (log2 ratio) -2
-1
0
1 at 185.057 kb on + strand at 185.109 kb on + strand at 185.189 kb on - strand at 185.597 kb on - strand, within Dshi_0182 at 185.678 kb on - strand at 185.801 kb on + strand, within Dshi_0183 at 185.801 kb on + strand, within Dshi_0183 at 185.844 kb on + strand, within Dshi_0183 at 186.169 kb on - strand, within Dshi_0183 at 186.425 kb on + strand, within Dshi_0184 at 186.627 kb on - strand, within Dshi_0184 at 186.741 kb on + strand at 186.749 kb on - strand at 186.749 kb on - strand at 186.855 kb on + strand, within Dshi_0185 at 186.952 kb on + strand, within Dshi_0185 at 186.952 kb on + strand, within Dshi_0185 at 186.977 kb on + strand, within Dshi_0185 at 187.004 kb on - strand, within Dshi_0185 at 187.065 kb on - strand, within Dshi_0185 at 187.140 kb on - strand, within Dshi_0185
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Tetracycline 0.006 mg/ml remove 185,057 + -0.9 185,109 + +1.7 185,189 - +0.7 185,597 - Dshi_0182 0.86 +0.7 185,678 - -2.0 185,801 + Dshi_0183 0.24 -0.8 185,801 + Dshi_0183 0.24 +0.7 185,844 + Dshi_0183 0.31 -1.3 186,169 - Dshi_0183 0.85 -0.3 186,425 + Dshi_0184 0.38 -2.3 186,627 - Dshi_0184 0.83 -0.5 186,741 + -1.9 186,749 - +0.1 186,749 - -2.0 186,855 + Dshi_0185 0.12 -1.2 186,952 + Dshi_0185 0.22 -0.2 186,952 + Dshi_0185 0.22 +0.5 186,977 + Dshi_0185 0.25 -2.3 187,004 - Dshi_0185 0.27 -2.5 187,065 - Dshi_0185 0.34 -1.9 187,140 - Dshi_0185 0.42 -0.1
Or see this region's nucleotide sequence