Experiment: m.b. Nickel (II) chloride 0.625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09130 and PGA1_c09140 overlap by 4 nucleotides PGA1_c09140 and PGA1_c09150 overlap by 4 nucleotides PGA1_c09150 and PGA1_c09160 are separated by 3 nucleotides PGA1_c09160 and PGA1_c09170 overlap by 4 nucleotides
PGA1_c09130: PGA1_c09130 - 8-amino-7-oxononanoate synthase BioF, at 938,722 to 939,861
_c09130
PGA1_c09140: PGA1_c09140 - dethiobiotin synthetase BioD, at 939,858 to 940,499
_c09140
PGA1_c09150: PGA1_c09150 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA, at 940,496 to 941,794
_c09150
PGA1_c09160: PGA1_c09160 - Uncharacterized protein conserved in bacteria, at 941,798 to 942,418
_c09160
PGA1_c09170: PGA1_c09170 - putative biotin synthesis protein BioC, at 942,415 to 943,203
_c09170
Position (kb) 940
941
942 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 939.528 kb on + strand, within PGA1_c09130 at 939.533 kb on + strand, within PGA1_c09130 at 939.541 kb on - strand, within PGA1_c09130 at 939.604 kb on - strand, within PGA1_c09130 at 939.634 kb on + strand, within PGA1_c09130 at 939.646 kb on + strand, within PGA1_c09130 at 939.661 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.696 kb on + strand, within PGA1_c09130 at 939.704 kb on - strand, within PGA1_c09130 at 939.719 kb on + strand, within PGA1_c09130 at 939.727 kb on - strand, within PGA1_c09130 at 939.807 kb on + strand at 939.807 kb on + strand at 939.812 kb on + strand at 939.815 kb on - strand at 939.820 kb on - strand at 939.829 kb on + strand at 939.945 kb on - strand, within PGA1_c09140 at 939.997 kb on + strand, within PGA1_c09140 at 940.023 kb on - strand, within PGA1_c09140 at 940.033 kb on - strand, within PGA1_c09140 at 940.084 kb on - strand, within PGA1_c09140 at 940.156 kb on + strand, within PGA1_c09140 at 940.252 kb on + strand, within PGA1_c09140 at 940.300 kb on + strand, within PGA1_c09140 at 940.308 kb on - strand, within PGA1_c09140 at 940.311 kb on + strand, within PGA1_c09140 at 940.362 kb on - strand, within PGA1_c09140 at 940.458 kb on + strand at 940.486 kb on - strand at 940.486 kb on - strand at 940.511 kb on - strand at 940.549 kb on + strand at 940.549 kb on + strand at 940.549 kb on + strand at 940.557 kb on - strand at 940.557 kb on - strand at 940.557 kb on - strand at 940.621 kb on + strand at 940.657 kb on - strand, within PGA1_c09150 at 940.725 kb on - strand, within PGA1_c09150 at 940.730 kb on - strand, within PGA1_c09150 at 940.921 kb on - strand, within PGA1_c09150 at 941.007 kb on - strand, within PGA1_c09150 at 941.007 kb on - strand, within PGA1_c09150 at 941.125 kb on + strand, within PGA1_c09150 at 941.125 kb on + strand, within PGA1_c09150 at 941.159 kb on + strand, within PGA1_c09150 at 941.159 kb on + strand, within PGA1_c09150 at 941.164 kb on + strand, within PGA1_c09150 at 941.410 kb on - strand, within PGA1_c09150 at 941.427 kb on + strand, within PGA1_c09150 at 941.472 kb on - strand, within PGA1_c09150 at 941.482 kb on + strand, within PGA1_c09150 at 941.484 kb on - strand, within PGA1_c09150 at 941.504 kb on + strand, within PGA1_c09150 at 941.512 kb on - strand, within PGA1_c09150 at 941.525 kb on + strand, within PGA1_c09150 at 941.525 kb on + strand, within PGA1_c09150 at 941.528 kb on - strand, within PGA1_c09150 at 941.545 kb on + strand, within PGA1_c09150 at 941.545 kb on + strand, within PGA1_c09150 at 941.553 kb on - strand, within PGA1_c09150 at 941.559 kb on - strand, within PGA1_c09150 at 941.610 kb on + strand, within PGA1_c09150 at 941.618 kb on - strand, within PGA1_c09150 at 941.618 kb on - strand, within PGA1_c09150 at 941.707 kb on + strand at 941.715 kb on - strand at 941.715 kb on - strand at 941.758 kb on - strand at 941.788 kb on + strand at 941.814 kb on - strand at 941.820 kb on - strand at 941.825 kb on - strand at 941.931 kb on + strand, within PGA1_c09160 at 941.986 kb on - strand, within PGA1_c09160 at 942.013 kb on - strand, within PGA1_c09160 at 942.077 kb on - strand, within PGA1_c09160 at 942.077 kb on - strand, within PGA1_c09160 at 942.115 kb on + strand, within PGA1_c09160 at 942.130 kb on - strand, within PGA1_c09160 at 942.242 kb on - strand, within PGA1_c09160 at 942.249 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.277 kb on + strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.285 kb on - strand, within PGA1_c09160 at 942.358 kb on + strand at 942.396 kb on + strand at 942.409 kb on + strand at 942.428 kb on - strand at 942.438 kb on - strand at 942.483 kb on - strand at 942.755 kb on - strand, within PGA1_c09170 at 942.756 kb on + strand, within PGA1_c09170 at 942.764 kb on + strand, within PGA1_c09170
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 0.625 mM remove 939,528 + PGA1_c09130 0.71 +0.0 939,533 + PGA1_c09130 0.71 +0.9 939,541 - PGA1_c09130 0.72 -1.9 939,604 - PGA1_c09130 0.77 +1.1 939,634 + PGA1_c09130 0.80 -0.3 939,646 + PGA1_c09130 0.81 +2.2 939,661 + PGA1_c09130 0.82 +0.5 939,696 + PGA1_c09130 0.85 +0.1 939,696 + PGA1_c09130 0.85 +0.7 939,696 + PGA1_c09130 0.85 -2.1 939,696 + PGA1_c09130 0.85 +1.7 939,696 + PGA1_c09130 0.85 +0.5 939,704 - PGA1_c09130 0.86 +0.2 939,719 + PGA1_c09130 0.87 +1.4 939,727 - PGA1_c09130 0.88 +2.5 939,807 + +0.7 939,807 + +1.1 939,812 + +1.1 939,815 - +0.1 939,820 - +1.1 939,829 + -0.3 939,945 - PGA1_c09140 0.14 +0.4 939,997 + PGA1_c09140 0.22 -0.3 940,023 - PGA1_c09140 0.26 +0.0 940,033 - PGA1_c09140 0.27 -0.7 940,084 - PGA1_c09140 0.35 +2.2 940,156 + PGA1_c09140 0.46 +2.1 940,252 + PGA1_c09140 0.61 -0.1 940,300 + PGA1_c09140 0.69 -0.2 940,308 - PGA1_c09140 0.70 -0.1 940,311 + PGA1_c09140 0.71 -0.8 940,362 - PGA1_c09140 0.79 +0.9 940,458 + +1.0 940,486 - -0.8 940,486 - +0.2 940,511 - +2.2 940,549 + +1.4 940,549 + +1.2 940,549 + +0.2 940,557 - +0.1 940,557 - -0.7 940,557 - +0.7 940,621 + -0.3 940,657 - PGA1_c09150 0.12 -1.9 940,725 - PGA1_c09150 0.18 +0.7 940,730 - PGA1_c09150 0.18 +1.5 940,921 - PGA1_c09150 0.33 -0.3 941,007 - PGA1_c09150 0.39 +0.5 941,007 - PGA1_c09150 0.39 -1.9 941,125 + PGA1_c09150 0.48 +1.2 941,125 + PGA1_c09150 0.48 -0.8 941,159 + PGA1_c09150 0.51 +0.3 941,159 + PGA1_c09150 0.51 -0.7 941,164 + PGA1_c09150 0.51 +0.9 941,410 - PGA1_c09150 0.70 +1.9 941,427 + PGA1_c09150 0.72 +1.2 941,472 - PGA1_c09150 0.75 -0.9 941,482 + PGA1_c09150 0.76 +1.3 941,484 - PGA1_c09150 0.76 -0.1 941,504 + PGA1_c09150 0.78 +0.7 941,512 - PGA1_c09150 0.78 +0.7 941,525 + PGA1_c09150 0.79 -0.9 941,525 + PGA1_c09150 0.79 -0.1 941,528 - PGA1_c09150 0.79 +1.1 941,545 + PGA1_c09150 0.81 -0.5 941,545 + PGA1_c09150 0.81 +3.2 941,553 - PGA1_c09150 0.81 -0.9 941,559 - PGA1_c09150 0.82 +1.1 941,610 + PGA1_c09150 0.86 +0.2 941,618 - PGA1_c09150 0.86 -0.3 941,618 - PGA1_c09150 0.86 +0.2 941,707 + +1.1 941,715 - +1.7 941,715 - +1.1 941,758 - -0.7 941,788 + -0.6 941,814 - -0.7 941,820 - +1.1 941,825 - +0.5 941,931 + PGA1_c09160 0.21 -0.3 941,986 - PGA1_c09160 0.30 +0.4 942,013 - PGA1_c09160 0.35 +2.2 942,077 - PGA1_c09160 0.45 +0.4 942,077 - PGA1_c09160 0.45 +1.5 942,115 + PGA1_c09160 0.51 +0.6 942,130 - PGA1_c09160 0.53 +2.0 942,242 - PGA1_c09160 0.71 -1.0 942,249 + PGA1_c09160 0.73 -0.3 942,277 + PGA1_c09160 0.77 +2.2 942,277 + PGA1_c09160 0.77 +1.2 942,277 + PGA1_c09160 0.77 -0.6 942,285 - PGA1_c09160 0.78 +1.4 942,285 - PGA1_c09160 0.78 -0.3 942,285 - PGA1_c09160 0.78 +0.3 942,358 + +1.1 942,396 + -0.9 942,409 + +0.7 942,428 - +0.0 942,438 - +1.2 942,483 - +0.1 942,755 - PGA1_c09170 0.43 +0.7 942,756 + PGA1_c09170 0.43 -1.3 942,764 + PGA1_c09170 0.44 -0.9
Or see this region's nucleotide sequence