Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1847 and Shewana3_1848 are separated by 44 nucleotides Shewana3_1848 and Shewana3_1849 overlap by 19 nucleotides Shewana3_1849 and hisH are separated by 29 nucleotides
Shewana3_1847: Shewana3_1847 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,189,352 to 2,190,011
_1847
Shewana3_1848: Shewana3_1848 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,190,056 to 2,190,829
_1848
Shewana3_1849: Shewana3_1849 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,190,811 to 2,191,548
_1849
Shewana3_1850: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,191,578 to 2,192,228
hisH
Position (kb)
2190
2191 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2189.292 kb on + strand at 2189.292 kb on + strand at 2189.332 kb on - strand at 2189.337 kb on - strand at 2189.337 kb on - strand at 2189.339 kb on - strand at 2189.575 kb on + strand, within Shewana3_1847 at 2189.640 kb on + strand, within Shewana3_1847 at 2189.642 kb on + strand, within Shewana3_1847 at 2189.996 kb on - strand at 2190.055 kb on - strand at 2190.406 kb on + strand, within Shewana3_1848 at 2190.633 kb on + strand, within Shewana3_1848 at 2190.634 kb on + strand, within Shewana3_1848 at 2190.641 kb on - strand, within Shewana3_1848 at 2190.778 kb on - strand at 2190.826 kb on + strand at 2190.981 kb on + strand, within Shewana3_1849 at 2190.989 kb on - strand, within Shewana3_1849 at 2191.094 kb on - strand, within Shewana3_1849 at 2191.651 kb on + strand, within hisH at 2191.714 kb on - strand, within hisH
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,189,292 + -1.8 2,189,292 + +2.6 2,189,332 - +0.1 2,189,337 - -0.9 2,189,337 - -1.1 2,189,339 - +0.1 2,189,575 + Shewana3_1847 0.34 -4.1 2,189,640 + Shewana3_1847 0.44 -4.1 2,189,642 + Shewana3_1847 0.44 -4.0 2,189,996 - -6.0 2,190,055 - +0.9 2,190,406 + Shewana3_1848 0.45 -5.3 2,190,633 + Shewana3_1848 0.75 -5.0 2,190,634 + Shewana3_1848 0.75 -4.5 2,190,641 - Shewana3_1848 0.76 -5.7 2,190,778 - -6.0 2,190,826 + -4.8 2,190,981 + Shewana3_1849 0.23 -6.1 2,190,989 - Shewana3_1849 0.24 -4.7 2,191,094 - Shewana3_1849 0.38 -6.7 2,191,651 + hisH Shewana3_1850 0.11 -3.6 2,191,714 - hisH Shewana3_1850 0.21 -4.1
Or see this region's nucleotide sequence